PXD067974-1

PXD067974 is an original dataset announced via ProteomeXchange.

Title	Exploring the Plasma Proteome Linked to Corpus Luteum Presence and Conception Mode Across Pregnancy Stages and Postpartum
Description	Observational data suggest that women conceiving without a corpus luteum are at higher risk of developing preeclampsia. While the underlying mechanisms remain unclear, the absence of corpus luteum-derived secretory products may be a contributing factor. This study investigates whether the plasma proteome differs between women who conceive with or without a corpus luteum and examines the relationship with mode of conception. Plasma samples from 12 participants were collected at three time points: first trimester, third trimester, and postpartum. The cohort included women who conceived unassisted (UC) after infertility, via artificial cycle frozen embryo transfer (AC FET), or natural cycle frozen embryo transfer (NC FET). 36 plasma protein samples were analyzed using mass spectrometry-based proteomics to compare the proteome of women who conceived with and without a corpus luteum, across different conception methods and pregnancy stages. In total, 528 proteins were quantified. No differentially expressed plasma proteins were identified between women with and without a corpus luteum. However, 15 proteins showed differential expression between UC and FET at all time points, with Bonferroni-corrected p<9.47 × 10E-5 and FC≥|2|. Several altered proteins, including PAPPA and ANG, were linked to preeclampsia. SERPINA7 was differentially detected when comparing time points within the unassisted conception method. No significant differences were detected between AC FET and NC FET. This pilot study revealed a unique proteomic signature associated with mode of conception. The findings suggest biologically plausible candidate proteins for further testing. Validation in larger cohorts or with alternative proteome analysis technologies is needed
HostingRepository	PRIDE
AnnounceDate	2025-10-07
AnnouncementXML	Submission_2025-10-07_10:31:49.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Pich
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-02 02:03:57	ID requested
⏵ 1	2025-10-07 10:31:50	announced

Chung B, Jonas W, Rostami F, Malik Z, Szaroszyk M, Levay M, Thackeray J, Ost M, Nowak S, Hegermann J, Wieland T, Pich A, Bengel FM, Bauersachs J, Wollert KC, Heineke J, Sch, ü, rmann A, Kempf T, Iron regulatory proteins secure iron availability in skeletal muscle to preserve exercise tolerance in heart failure. Cardiovasc Res, ():(2025) [pubmed]

10.1093/cvr/cvaf173;

submitter keyword: frozen embryo transfer, mode of conception, unassisted conception,corpus luteum, in vitro fertilization, proteome

Andreas Pich
contact affiliation	Hannover Medical School Carl-Neuberg-Str. 1 30625 Hannover Germany
contact email	pich.andreas@mh-hannover.de
lab head
Andreas Pich
contact affiliation	Hannover Medical School Core Facility Proteomics
contact email	pich.andreas@mh-hannover.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD067974

PRIDE project URI

• PRIDE
  1. PXD067974
     1. Label: PRIDE project
     2. Name: Exploring the Plasma Proteome Linked to Corpus Luteum Presence and Conception Mode Across Pregnancy Stages and Postpartum