PXD067955-1

PXD067955 is an original dataset announced via ProteomeXchange.

Title	Florigen activation complex forms via multifaceted assembly in Arabidopsis
Description	'Florigen', encoded by FT genes, is synthesized in leaves and transported to the shoot apical meristem (SAM) to induce flower development. At the SAM, 14-3-3 proteins are proposed to act as receptors for FT and to mediate the indirect interaction between FT and the bZIP transcription factor FD to form the 'florigen activation complex' (FAC) that activates transcription of flowering genes. We demonstrate a different mechanism of FAC assembly, diverse functions for the 14-3-3s within the complex and an unexpected spatiotemporal distribution of the FAC. We show that FT is not recruited by 14-3-3 alone, but that it interacts with the DNA−FD−14-3-3 complex through two interfaces, one of which binds DNA via the unstructured C-terminus of FT. Also, we find that interaction of 14-3-3s with the phosphorylated FD C-terminus reduces liquid-phase condensation of the intrinsically disordered FD protein allowing it to bind DNA, and that the 14-3-3s strengthen DNA binding of FD by promoting dimerization, which ultimately results in the recruitment of FT. Unexpectedly, we also discovered that after FT movement to the shoot apex, FT and FD are co-transcribed in young floral primordia forming a boundary with the suppressed bract and allowing formation of the FAC during the first stages of floral development. Our studies propose a new mechanism by which the florigen FT transcriptional complex is formed, and indicate distinct functions for the complex during SAM and floral primordium development.
HostingRepository	PRIDE
AnnounceDate	2025-09-02
AnnouncementXML	Submission_2025-09-02_07:42:43.350.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Christina Stolze
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-01 09:50:53	ID requested
⏵ 1	2025-09-02 07:42:43	announced

Dataset with its publication pending

submitter keyword: Florigen Activation Complex, IP-MS, 14-3-3, phosphorylation, Flowering,FT, FD

Hirofumi Nakagami
contact affiliation	Basic Immune System of Plants / Protein Mass SpectrometryMax Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact email	nakagami@mpipz.mpg.de
lab head
Sara Christina Stolze
contact affiliation	Plant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne
contact email	stolze@mpipz.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD067955

PRIDE project URI

• PRIDE
  1. PXD067955
     1. Label: PRIDE project
     2. Name: Florigen activation complex forms via multifaceted assembly in Arabidopsis