PXD067952-1

PXD067952 is an original dataset announced via ProteomeXchange.

Title	Beyond difficile: three novel toxin B-producing Clostridioides species from human patients with diarrhea (exoproteome)
Description	Clostridioides difficile infection (CDI), caused by strains producing toxin B (TcdB), poses a significant global health threat. While C. difficile exhibits substantial diversity, functional studies have focused on a limited number of isolates, overlooking other genomospecies within the genus. We describe five isolates from patients suspected of having CDI who tested negative for the PaLoc marker gene tcdC. Through genomic, proteomic, and phenotypic analyses, we demonstrate that they correspond to three novel toxin-producing species, designated as Clostridioides cryptodifficilis sp. nov., Clostridioides divergens sp. nov., and Clostridioides subdifficilis sp. nov. These species are distinguished by unique MALDI-ToF signatures, metabolic capabilities, and genomic and proteomic architectures, underscoring their clear taxonomic and functional divergence from C. difficile. They secrete functionally active TcdB7 or TcdB11, as demonstrated by cytotoxicity assays in cultured cells and in vivo using the mouse ileal loop model, implicating them in disease pathology, albeit with lower virulence than C. difficile. Our findings expand the known diversity of TcdB-producing Clostridioides and have direct implications for diagnostics, surveillance, and clinical management of diarrheal diseases.
HostingRepository	PRIDE
AnnounceDate	2026-05-06
AnnouncementXML	Submission_2026-05-05_23:34:25.961.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Clostridioides sp.; NCBI TaxID: NEWT:1972855;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-01 06:25:21	ID requested
⏵ 1	2026-05-05 23:34:26	announced

10.1080/22221751.2026.2671473;

submitter keyword: omics, Clostridioides sp. nov.,C. difficile, TcdB, taxonomy, pathogenesis

Dörte Becher
contact affiliation	Department of Microbial Proteomics, University of Greifswald, Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067952
     1. Label: PRIDE project
     2. Name: Beyond difficile: three novel toxin B-producing Clostridioides species from human patients with diarrhea (exoproteome)