PXD067912 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | P300 proximity labeling in human iPSCs |
| Description | This dataset presents the results of proximity-dependent biotinylation targeting the transcriptional coactivator p300 in human induced pluripotent stem cells (hiPSCs). We endogenously tagged p300 with the biotin ligase miniTurboID, to enable labeling of proximal proteins and thus proteins locatd at enhancers. hiPSCs were cultured in two different conditions, either StemFit or mTeSR medium, to assess how media composition influences the p300 proximal proteome. Following biotin labeling, proteins were enriched via streptavidin pulldown and analyzed by LC-MS/MS. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-07 |
| AnnouncementXML | Submission_2026-08-07_03:44:46.381.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | S Stelloo |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-31 09:33:26 | ID requested | |
| ⏵ 1 | 2026-08-07 03:44:47 | announced | |
Publication List
| 10.1016/j.stemcr.2026.103023; |
| Alejo-Vinogradova MT, Str, ΓΈ, m YE, Lamers LA, Chang YW, Hendriksen MJ, Moosajee M, Vermeulen M, Stelloo S, From stem cells to somites: Revealing genetic and exogenous factors of human embryogenesis. Stem Cell Reports, ():103023(2026) [pubmed] |
Keyword List
Contact List
| Prof. Michiel |
|---|
| contact affiliation | Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, 6525 GA Nijmegen, The Netherlands |
| contact email | michiel.vermeulen@ru.nl |
| lab head | |
| S Stelloo |
|---|
| contact affiliation | Department of Urology, Radboud University Medical Center (RUMC), Nijmegen, The Netherlands |
| contact email | stelloo@science.ru.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD067912 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067912
- Label: PRIDE project
- Name: P300 proximity labeling in human iPSCs
