PXD067868-1

PXD067868 is an original dataset announced via ProteomeXchange.

Title	Mapping of the hSOX10 protein interactome in human melanoma
Description	The transcription factor SOX10 is a central regulator of melanoma biology, influencing tumor initiation, progression, phenotypic plasticity, and therapeutic resistance. However, the molecular mechanisms underlying these diverse functions remain incompletely understood. To elucidate the protein-protein interactions (PPIs) that mediate SOX10 activity in melanoma, we mapped the human SOX10 (hSOX10) interactome for the first time in human melanoma cells (A375 line). Given the challenges of capturing transient and weak transcription factor interactions, we employed miniTurbo (mT) proximity-dependent biotinylation coupled with mass spectrometry (MS). Stable A375 cell lines expressing N- and C-terminal mT-tagged hSOX10 fusion proteins at physiologically relevant levels were generated, enabling unbiased biotinylation and MS-based identification of candidate interacting proteins. This approach revealed 847 melanoma-enriched candidate interactors, including 213 high-confidence hits. These included both known hSOX10 partners and previously unidentified putative interactors. Functional annotation of our hSOX10 interactome highlighted associations with chromatin remodeling, transcriptional regulation, SUMOylation, and DNA damage response pathways. Western blot validation of select interactors supported the robustness of our dataset. This first comprehensive map of the hSOX10 interactome in melanoma provides a critical foundation for future investigations into SOX10-driven transcriptional networks and their potential as therapeutic targets and biomarkers in melanoma.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_16:29:52.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dhaval Bhatt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-28 19:24:15	ID requested
⏵ 1	2026-06-14 16:29:53	announced

10.1021/acs.jproteome.5c00852;

Newsom-Stewart CM, Bhatt DP, Major MB, Kaufman CK, Mapping of the hSOX10 Proximal Protein Interactome in Human Melanoma. J Proteome Res, 25(6):2649-2668(2026) [pubmed]

submitter keyword: interactome mapping, mass spectrometry, miniTurbo, melanoma, proximity-based biotinylation, protein-protein interactions,hSOX10

Charles K. Kaufman
contact affiliation	Division of Medical Oncology and Department of Developmental Biology, Washington University in St. Louis, St. Louis, MO, USA. Siteman Cancer Center, Washington University in St. Louis, St. Louis, MO, USA.
contact email	ckkaufman@wustl.edu
lab head
Dhaval Bhatt
contact affiliation	Washington University in St. Louis
contact email	dhaval.bhatt@wustl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067868
     1. Label: PRIDE project
     2. Name: Mapping of the hSOX10 protein interactome in human melanoma