PXD067856-1

PXD067856 is an original dataset announced via ProteomeXchange.

Title	Human RNA ligase 1 as a novel regulator of ribosome function and translation under oxidative stress
Description	Human RNA ligase 1 (Rlig1) is a recently identified human 5’-3’ RNA ligase essential for maintaining 28S rRNA integrity and promoting cell survival under oxidative stress. Although the enzymatic activity of Rlig1 implies a role in RNA maintenance or repair, its broader molecular context remains insufficiently characterised. Here we identify cellular interactors of Rlig1 using affinity enrichment coupled to mass spectrometry in a HEK293 Rlig1-KO model. Our approach revealed several RNA and DNA surveillance, degradation and repair proteins as well as a diverse set of RNA binding and RNA processing enzymes as potential interactors. Notably, ribosomal proteins were highly enriched, suggesting a link between Rlig1 and the translational machinery.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-25_06:57:17.383.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stumpf
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-28 13:02:08	ID requested
⏵ 1	2026-05-25 06:57:17	announced

Dataset with its publication pending

submitter keyword: Q-Exactive HF, Angel2, in-solution digest, AMPylation, human, DIS3, Rlig1, CLP1, RNA ligase, HEK293, DIA, ribosome, Enrichment, oxidative stress, Exosome,AP-MS, RNA repair

Florian Stengel
contact affiliation	Department of Biology, University of Konstanz, Germany
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stumpf
contact affiliation	University of Konstanz
contact email	florian.stumpf@uni-konstanz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067856
     1. Label: PRIDE project
     2. Name: Human RNA ligase 1 as a novel regulator of ribosome function and translation under oxidative stress