⮝ Full datasets listing
PXD067851-1
PXD067851 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic Mapping Reveals how Lrp2 Interacts with the Endocytic Machine |
| Description | LRP2 (Megalin or Low-density lipoprotein-related receptor 2), together with Cubilin and Amnionless, are responsible for binding and internalizing a wide range of nutrients and toxins from the kidney’s glomerular filtrate by endocytosis. In accordance, Lrp2 deletion or mutation results in loss of these ligands into the urine. Yet Lrp2 is essential not only for receptor-mediated, but also for fluid-phase endocytosis, implicating a broader role beyond ligand binding. To identify the linkage of Lrp2 and endocytosis, we engineered Lrp2-APEX2 expressing mice and performed biotinylation in vivo to label Lrp2’s cytoplasmic partners. We demonstrated the specificity and sensitivity of this technique by mass spectrometric identification of biotinylated proteins from the kidney lysate and by immunostaining kidney sections. We identified critical endocytic regulators interacting with Lrp2, but also many proteins functionally associated with endocytosis but not already known to interact with Lrp2. These data suggest that Lrp2 plays a central role in organizing apical membranes through PDZ domain proteins and engages with regulators and molecular motors during endocytosis. These interactions dissociate in the absence of Lrp2. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-03 |
| AnnouncementXML | Submission_2026-03-03_13:51:45.389.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Hediye Erdjument-Bromage |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-28 11:56:22 | ID requested | |
| ⏵ 1 | 2026-03-03 13:51:45 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Megalin, LRP2, Endocytosis, Microvilli, endosomes, endosytosis, proximity labeling,Apex2 enzyme, Cubilin, Amnionless, PDZ domain proteins, DatasetType:Proteomics |
Contact List
| Dr. Thomas A. Neubert | |
|---|---|
| contact affiliation | New York University School of Medicine |
| contact email | Thomas.Neubert@med.nyu.edu |
| lab head | |
| Dr. Jonathan Barasch | |
| contact affiliation | Columbia University |
| contact email | jmb4@cumc.columbia.edu |
| lab head | |
| Hediye Erdjument-Bromage | |
| contact affiliation | New York University School of Medicine |
| contact email | Hediye.Bromage@med.nyu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098968/ |
