PXD067843-1

PXD067843 is an original dataset announced via ProteomeXchange.

Title	Secretome of hTERT-Immortalized Human Mesenchymal Stem Cells
Description	The secretome of mesenchymal stromal cells (MSCs) can efficiently stimulate regener-ation and therefore is a tempting remedy for “cell-free cellular therapy”. However, the usage of primary MSC cultures as secretome-producers for translation studies has ob-vious obstacles, including rapid aging of MSC cultures, the need for a large number of verified donors and donor-to-donor variability of secretome content. MSCs immortal-ization allows to overcome those limitations and to obtain secretome-producing cul-tures with prolonged lifetime. However, the efficacy and safety of such secretomes are critical issues, which limit their usage as therapeutic agents. In this study we have tested in large detail how the immortalization of MSC cultures affects the content, bi-ological activity and safety of their secretome. MSCs immortalization via overexpres-sion of human TERT gene does not significantly alter the qualitative and quantitative composition of their secretome or its activity according to the results of proteomic analysis, ELISA, qPCR and functional tests in vitro. Moreover, we have demonstrated that the secretome of immortalized MSCs does not contain detectable amounts of te-lomerase and does not possess any transforming activity. Altogether, our data suggest that immortalized MSC cultures may become a reliable source for obtaining standard-ized active secretome in large-scale quantities for clinical use.The secretome of mesenchymal stromal cells (MSCs) can efficiently stimulate regener-ation and therefore is a tempting remedy for “cell-free cellular therapy”. However, the usage of primary MSC cultures as secretome-producers for translation studies has ob-vious obstacles, including rapid aging of MSC cultures, the need for a large number of verified donors and donor-to-donor variability of secretome content. MSCs immortal-ization allows to overcome those limitations and to obtain secretome-producing cul-tures with prolonged lifetime. However, the efficacy and safety of such secretomes are critical issues, which limit their usage as therapeutic agents. In this study we have tested in large detail how the immortalization of MSC cultures affects the content, bi-ological activity and safety of their secretome. MSCs immortalization via overexpres-sion of human TERT gene does not significantly alter the qualitative and quantitative composition of their secretome or its activity according to the results of proteomic analysis, ELISA, qPCR and functional tests in vitro. Moreover, we have demonstrated that the secretome of immortalized MSCs does not contain detectable amounts of te-lomerase and does not possess any transforming activity. Altogether, our data suggest that immortalized MSC cultures may become a reliable source for obtaining standard-ized active secretome in large-scale quantities for clinical use.
HostingRepository	PRIDE
AnnounceDate	2025-10-27
AnnouncementXML	Submission_2025-10-26_17:09:46.904.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elizaveta Chechekhina
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-28 08:40:01	ID requested
⏵ 1	2025-10-26 17:09:48	announced

Karagyaur M, Primak A, Basalova N, Monakova A, Tolstoluzhinskaya A, Kulebyakina M, Chechekhina E, Skryabina M, Grigorieva O, Chechekhin V, Yakovleva T, Turilova V, Shagimardanova E, Gazizova G, Vigovskiy M, Kulebyakin K, Sysoeva V, Dyachkova U, Dzhauari S, Bozov K, Popov V, Akopyan Z, Efimenko A, Kalinina N, Tkachuk V, Safety and Regenerative Properties of Immortalized Human Mesenchymal Stromal Cell Secretome. Int J Mol Sci, 26(19):(2025) [pubmed]

10.3390/ijms26199322;

submitter keyword: hTERT, mesenchymal stem cells,human, secretome

Maxim N. Karagyaur
contact affiliation	Lomonosov Moscow State University
contact email	m.karagyaur@mail.ru
lab head
Elizaveta Chechekhina
contact affiliation	Moscow State University
contact email	voynovaes.pharm@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD067843

PRIDE project URI

• PRIDE
  1. PXD067843
     1. Label: PRIDE project
     2. Name: Secretome of hTERT-Immortalized Human Mesenchymal Stem Cells