PXD067765 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Unveiling Dopamine and Met-Enkephalin Dynamics: Simultaneous Co-Detection in Rat Striatum |
| Description | Project description Fast -scan cyclic voltammetry was coupled with carbon microelectrodes for co-detection of dopamine and met-enkephalin at single recording sites in rat striatal slices. The measurements used a specifically designed voltammetric waveform to minimize sensitivity to dopamine, maximize sensitivity to enkephalin, and to minimize biofouling. Both neurotransmitter (dopamine) and neuropeptide (enkephalin) release scaled with stimulation duration. Enkephalin dynamics displayed a unique biphasic profile with a significant latency to peak that occurred ~30 seconds after stimulation. The data suggests a sphere of influence that is ~3x larger than that of dopamine and that is consistent with multiple forms of the peptide released at once. Signals were validated by mass spectrometry of collected striatal relesates. The findings provide direct evidence to support widely held assumptions regarding neuropeptide release, and they demonstrate how these signaling molecules can affect distinct cellular populations in striatum, even when recorded at the same site. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-22 |
| AnnouncementXML | Submission_2025-12-21_16:33:01.976.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD067765 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Elena Romanova |
| SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116; |
| ModificationList | monohydroxylated residue |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-26 16:55:39 | ID requested | |
| ⏵ 1 | 2025-12-21 16:33:03 | announced | |
Publication List
| Berger JM, Todorov J, Turner KM, McCarty GS, Romanova EV, Sweedler JV, Sombers LA, Unveiling Dopamine and Met-Enkephalin Dynamics: Simultaneous Co-Detection in Rat Striatum. Anal Chem, 97(45):25032-25046(2025) [pubmed] |
| 10.6019/PXD067765; |
| 10.1021/acs.analchem.5c03599; |
Keyword List
| submitter keyword: Proenkephalin, Mass Spectrometry, Solid-Phase Extraction, Opioid Peptide, Carbon-Fiber Microelectrode,Fast-Scan Cyclic Voltammetry, Mesolimbic Pathway |
Contact List
| Jonathan V. Sweedler |
|---|
| contact affiliation | Department of Chemistry University of Illinois 71 RAL, Box 63-5 600 South Mathews Avenue Urbana, IL 61801 |
| contact email | jsweedle@illinois.edu |
| lab head | |
| Elena Romanova |
|---|
| contact affiliation | University of Illinois |
| contact email | romanova@illinois.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD067765 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067765
- Label: PRIDE project
- Name: Unveiling Dopamine and Met-Enkephalin Dynamics: Simultaneous Co-Detection in Rat Striatum
