PXD067726-1

PXD067726 is an original dataset announced via ProteomeXchange.

Title	TMT-based quantitative proteomic assessment of Vicia sativa induced neurotoxicity by β-cyano-L-alanine and γ-glutamyl-β-cyano-L-alanine in SH-SY5Y cells
Description	β-Cyano-L-alanine (BCA) and γ-glutamyl-β-cyano-L-alanine (GBCA) are the primary antinutritional compounds in Vicia sativa, a high-protein, drought-tolerant legume. While neurotoxicity in monogastric animals has been observed, its molecular basis remains largely unknown. In this study, we optimised an in vitro assay using retinoic acid-differentiated SH-SY5Y human neuroblastoma cells to assess BCA and GBCA toxicity and applied TMT-based quantitative proteomics to identify dysregulated proteins. BCA treatment affected proteins involved in DNA damage, translation, and oxidative stress, many of which are associated with neurodegenerative diseases such as amyotrophic lateral sclerosis and Alzheimer’s disease, as well as various cancers. In contrast, GBCA disrupted proteins linked to mitosis, cell cycle regulation, and apoptosis pathways. Interestingly, the absence of overlapping dysregulated proteins between BCA- and GBCA-treated cells suggests they induce neurotoxicity via distinct mechanisms. These findings offer new insights into the molecular toxicity of V. sativa toxins and their implications for animal feed safety
HostingRepository	PRIDE
AnnounceDate	2026-01-19
AnnouncementXML	Submission_2026-01-18_17:13:08.319.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karthik Shantharam kamath
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-26 00:08:12	ID requested
⏵ 1	2026-01-18 17:13:09	announced

10.1038/s41598-025-30121-2;

Riley S, Nguyen V, Bhattacharjee R, Ng PQ, Ritchie T, Kamath KS, Fisk I, Gecz J, Kumar R, Searle IR, -cyano-L-alanine in SH-SY5Y cells. Sci Rep, 16(1):510(2025) [pubmed]

submitter keyword: Neurotoxicity, Vicia sativa, TMT-based proteomics, SH-SY5Y cells

Dr Iain Searle
contact affiliation	School of Biological Sciences, The University of Adelaide, Adelaide, South Australia, Australia
contact email	iain.searle@adelaide.edu.au
lab head
Karthik Shantharam kamath
contact affiliation	Macquarie university
contact email	karthikskamath@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067726
     1. Label: PRIDE project
     2. Name: TMT-based quantitative proteomic assessment of Vicia sativa induced neurotoxicity by β-cyano-L-alanine and γ-glutamyl-β-cyano-L-alanine in SH-SY5Y cells