PXD067665 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic Profiling of Oxidative Stress Response Proteins Using Methionine Sulfoxide-Inspired ABPP Probes |
| Description | Methionine oxidation is a significant signature of oxidative stress. In nature, oxidative stress from reactive oxygen species or reactive chlorine species (ROS/RCS) serves as a fundamental defense mechanism against pathogenic sources. However, previous tools for studying methionine oxidation have depended on sulfoxide reduction strategies, making them unsuitable for use under oxidative stress conditions. Moreover, no ABPP tools have been reported for studying the activity of cellular methionine sulfoxide reductases. In this paper, we present a cysteine-targeted chemical probe inspired by methionine sulfoxide for profiling oxidative stress response proteins, including methionine sulfoxide reductases. Our probe efficiently labels nucleophilic cysteines of methionine sulfoxide reductases and functions as an activity-based protein profiling tool. The probe demonstrated good reactivity and stability, making it applicable to both in vitro and in vivo experiments under oxidative stress conditions. We showed that our probe labeled oxidative stress-related proteins in E. coli under hypochlorite stress. Finally, we demonstrated that our ABPP probe offers complementary cysteine reactivity profiles compared to conventional probes, efficiently identifying proteins such as DJ-1 superfamily members that are poorly captured by iodoacetamide-alkyne. We believe that our probe can be used to reveal the biological mechanisms by which bacteria sense strong oxidants and endure oxidative stress, such as that encountered with neutrophils. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-09 |
| AnnouncementXML | Submission_2026-02-08_17:54:07.482.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Seungmin Ahn |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-24 19:53:46 | ID requested | |
| ⏵ 1 | 2026-02-08 17:54:08 | announced | |
Publication List
| 10.1021/jacsau.5c01237; |
| Ahn S, Kee JM, Proteomic Profiling of Oxidative Stress Response Proteins with a Methionine Sulfoxide-Inspired Activity-Based Probe. JACS Au, 6(1):310-318(2026) [pubmed] |
Keyword List
| submitter keyword: Ecoli,DJ-1,MethionineSulfoxideReductase,SO-acetylene,CysteineABPP |
Contact List
| Jung-Min Kee |
|---|
| contact affiliation | Department of Chemistry, Ulsan National Institute of Science and Technology |
| contact email | jmkee@unist.ac.kr |
| lab head | |
| Seungmin Ahn |
|---|
| contact affiliation | Ulsan National Institute of Science and Technology (UNIST) |
| contact email | asm8430@naver.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067665
- Label: PRIDE project
- Name: Proteomic Profiling of Oxidative Stress Response Proteins Using Methionine Sulfoxide-Inspired ABPP Probes
