PXD067632-1

PXD067632 is an original dataset announced via ProteomeXchange.

Title	Target antigen-displaying extracellular vesicles boost CAR T cell efficacy in neuroblastoma
Description	GPC2 and GD2 are validated CAR T cell targets in neuroblastoma, but durable responses remain limited. We profiled the surfaceome of neuroblastoma-derived extracellular vesicles (EVs) and assessed their impact on CAR T cell function. Neuroblastoma EVs displayed GPC2 and GD2, with minimal PD-L1, and were detected in blood from tumor-bearing mice and patients. These EVs directly activated paired CAR T cells, suggesting a role for a peripheral source of CAR antigen. To exploit this therapeutically, we engineered non-tumor-derived GPC2+ synthetic EVs (SyntEVs) as CAR T cell enhancers and armored them with either albumin- or GD2-binding domains. In mice harboring human neuroblastomas, serial infusion of armored SyntEVs following GPC2 CAR T cells enhanced tumor control by boosting peripheral CAR T cell persistence. Moreover, GD2-targeting SyntEVs decorated low-antigen tumor cells with GPC2, circumventing antigen downregulation. This SyntEV platform offers a versatile system to address the therapeutic limitations of CAR T cells in solid tumors.
HostingRepository	PRIDE
AnnounceDate	2026-01-29
AnnouncementXML	Submission_2026-01-29_14:31:13.243.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hossein Fazelinia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-22 21:12:32	ID requested
⏵ 1	2026-01-29 14:31:14	announced

Giudice AM, Matlaga S, Roth SL, Pascual-Pasto G, Sch, ü, rch PM, Rouin G, McIntyre B, Grothusen GP, Cresswell-Clay E, Shraim R, Groff D, Zecchino V, Lombardi S, Martinez D, Spruce LA, Brown EM, Fazelinia H, Henrickson SE, Nance J, Reynolds CP, Bosse KR, Target antigen-displaying extracellular vesicles boost CAR T cell efficacy in cell and mouse models of neuroblastoma. Sci Transl Med, 17(825):eads4214(2025) [pubmed]

10.1126/scitranslmed.ads4214;

submitter keyword: Neuroblastoma,CAR T Cell Targets, Extracellular Vesicles

Kristopher Bosse
contact affiliation	Ruth and Tristram Colket Jr. Translational Research Building Room 3050 3501 Civic Center Blvd Philadelphia, PA 19104
contact email	bossek@chop.edu
lab head
Hossein Fazelinia
contact affiliation	Children's Hospital of Philadelphia
contact email	fazeliniah@email.chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD067632

PRIDE project URI

• PRIDE
  1. PXD067632
     1. Label: PRIDE project
     2. Name: Target antigen-displaying extracellular vesicles boost CAR T cell efficacy in neuroblastoma