PXD067631 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Stimulus-specific Immune Modulation by SARS-CoV-2 Nucleocapsid Correlates with Severe COVID-19 |
| Description | A significant proportion of patients with COVID-19 disease, caused by SARS-CoV-2, develop acute respiratory distress syndrome characterized by pro-inflammatory cytokine secretion and dampened IFN-mediated antiviral response. To uncover the mechanisms by which SARS-CoV-2 drives this dysregulation, we conducted transcriptomic profiling of N-expressing monocyte-derived macrophages treated with different stimuli that activates several pattern recognition receptors. To capture differences in immunoinflammatory responses to different SARS-CoV-2 Nucleocapsids, we performed a comparative proteomics analysis between the hyper inflammatory Delta N protein and less inflammatory Omicron N protein. Both Nucleocapsids expressed in monocyte-derived macrophages were pulled down for affinity purification mass spectrometry (APMS) and their differential protein interactors were identified; revealing Delta N interacts more strongly with stress granule proteins compared to Omicron N, which may explain Delta N pro-inflammatory phenotype. This project was a collaboration between the labs of Dr. Melody Li, Dr. Alexander Hoffmann, and Dr. Mehdi Bouhaddou at UCLA. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-26 |
| AnnouncementXML | Submission_2026-06-25_16:49:09.004.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mehdi Bouhaddou |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: NEWT:2697049; |
| ModificationList | acetylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-22 21:12:02 | ID requested | |
| ⏵ 1 | 2026-06-25 16:49:09 | announced | |
Publication List
| Yao Z, Alvarez PA, Chavez C, Delgado Y, Kaushal P, Austin D, Li Q, Yu Y, Zaiss AK, Arumugaswami V, Ding Q, Hsu JJ, Damoiseaux R, Bouhaddou M, Hoffmann A, Li MMH, SARS-CoV-2 nucleocapsid induces hyperinflammation and vascular leakage through the Toll-like receptor signaling axis in macrophages. bioRxiv, ():(2025) [pubmed] |
| 10.1101/2025.08.28.672752; |
Keyword List
| submitter keyword: SARS-CoV-2, macrophages, NF-kB pathways, Nucleocapsid (N), pro-inflammatory responses |
Contact List
| Mehdi Bouhaddou |
|---|
| contact affiliation | UCLA |
| contact email | mehdibouhaddou@gmail.com |
| lab head | |
| Mehdi Bouhaddou |
|---|
| contact affiliation | UCLA |
| contact email | bouhaddoulab@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD067631 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067631
- Label: PRIDE project
- Name: Stimulus-specific Immune Modulation by SARS-CoV-2 Nucleocapsid Correlates with Severe COVID-19
