PXD067629 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ligand-dependent conformational dynamics of the α2A-adrenergic receptor |
| Description | Biased agonists targeting the α2A-adrenergic receptor (α2AAR) have therapeutic potential by preferentially engaging G protein signaling over β-arrestin pathways. Here, we used hydrogen-deuterium exchange mass spectrometry (HDX-MS) to compare α2AAR conformational dynamics across apo, agonist-bound, and GoA-coupled states. We analyzed HDX-MS changes induced by norepinephrine (NorEpi), the endogenous full agonist; dexmedetomidine (Dex), a clinically used full agonist that activates both Gi/o and β-arrestin signaling; and PS75, a Gi/o-biased partial agonist. By quantifying ligand-dependent differences in deuterium uptake and local dynamics, we aimed to define ligand-specific conformational ensembles. These findings provide mechanistic insight into partial agonism and suggest potential implications for biased signaling. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-25 |
| AnnouncementXML | Submission_2026-02-25_15:21:04.001.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Donghoon Ahn |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Xevo G2-XS QTof |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-22 20:34:05 | ID requested | |
| ⏵ 1 | 2026-02-25 15:21:04 | announced | |
Publication List
Keyword List
Contact List
| Ka Young |
|---|
| contact affiliation | School of Pharmacy, Sungkyunkwan University, Suwon, 16419, Republic of Korea |
| contact email | kychung2@skku.edu |
| lab head | |
| Donghoon Ahn |
|---|
| contact affiliation | School of Pharmacy, Sungkyunkwan University, Suwon, 16419, Republic of Korea |
| contact email | jnsd5102@skku.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067629
- Label: PRIDE project
- Name: Ligand-dependent conformational dynamics of the α2A-adrenergic receptor
