PXD067626 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In situ Proteomics of Brain Cilia Unveils Molecular Mechanisms of Cilia-Synapse Interactions |
| Description | Neuronal cilia have emerged as pivotal signaling hubs; yet the ciliary molecules that organize signaling modalities and communication with neighboring synapses remain elusive. To map the brain cilia proteome, we engineered an Arl13b-TurboID mouse that enabled robust cilia-specific biotinylation in all cell types. Comparative quantitative proteomics revealed that neuronal and kidney cilia share less than half of their proteome. The brain cilia proteome encompasses synaptic proteins, transporters, adhesion molecules, and neurotransmitter receptors. Surprisingly, several signaling and adhesion molecules localize on neuronal cilia in discrete patterns, which are actively established. Mapping the NMDA receptor GluN1 at 25 nm resolution in the mouse cortex revealed a close, non-random association between ciliary GluN1 molecules and neighboring glutamatergic synapses. Cilia may thus eavesdrop on synaptic communication by re-deploying parts of the synaptic apparatus. Our study highlights the diversity and specialization of mammalian ciliary proteomes and indicates that nanoscopic organization may endow neuronal cilia with extrasynaptic functions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-08 |
| AnnouncementXML | Submission_2026-06-08_01:47:07.566.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD067626 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Marian Kalocsay |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480; Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-22 18:23:33 | ID requested | |
| ⏵ 1 | 2026-06-08 01:47:08 | announced | |
Publication List
| 10.6019/PXD067626; |
| Chang CH, Trinh VN, Novak SW, Lokesh NR, Montecinos CK, Boassa D, Pownall ME, Kalocsay M, Nachury MV, In situ proteomics unveils specialized domains for extrasynaptic signaling on neuronal cilia. Sci Adv, 12(23):eaed5548(2026) [pubmed] |
| 10.1126/sciadv.aed5548; |
Keyword List
| submitter keyword: expansion microscopy,neuronal cilia, proximity proteomics, cilia-synapse interaction, synaptic signaling |
Contact List
| Maxence Nachury |
|---|
| contact affiliation | Professor, Ophtalmology, School of Medicine, University of California, Sam Fancisco (UCSF) |
| contact email | Maxence.Nachury@ucsf.edu |
| lab head | |
| Marian Kalocsay |
|---|
| contact affiliation | The University of Texas MD Anderson Cancer Center
Department of Experimental Radiation Oncology |
| contact email | mkalocsay@mdanderson.org |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD067626 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067626
- Label: PRIDE project
- Name: In situ Proteomics of Brain Cilia Unveils Molecular Mechanisms of Cilia-Synapse Interactions
