PXD067614-1

PXD067614 is an original dataset announced via ProteomeXchange.

Title	Cooperative Aldehyde Chemistry Maps an Orthogonal Lysine Reactivity Landscape
Description	Reactive metabolites are often associated with nonspecific protein damage, if harnessed cooperatively they could be repurposed into precision tools for targeted labeling, redefining how we probe and manipulate protein function in complex biological systems. Here, we introduce a bioinspired cooperative metabolite strategy that harnesses the synergistic chemistry of malondialdehyde (MDA) and monoaldehydes to generate highly reactive intermediates that selectively modify lysine residues across proteins and proteomes. Dose-dependent chemoproteomic profiling of proteins modified by MDA-monoaldehyde complexes reveals 245 peptides and 167 unique proteins commonly modified in all doses. Among these, nearly 100 proteins feature a single modification at only hyperreactive lysine residue, indicating distinct microenvironmental features that enhance lysine reactivity and selectivity toward cooperative metabolite modification. Captured proteins were found to be linked to disease pathways and environmental exposures, revealing previously unrecognized patterns of metabolic susceptibility.
HostingRepository	PRIDE
AnnounceDate	2026-04-01
AnnouncementXML	Submission_2026-04-01_03:24:53.397.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Riley Hughes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-22 09:55:33	ID requested
⏵ 1	2026-04-01 03:24:53	announced

Dataset with its publication pending

submitter keyword: None

Monika Raj
contact affiliation	Emory University, Department of Chemistry
contact email	monika.raj@emory.edu
lab head
Riley Hughes
contact affiliation	Emory University
contact email	riley.hughes@emory.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD067614

PRIDE project URI

• PRIDE
  1. PXD067614
     1. Label: PRIDE project
     2. Name: Cooperative Aldehyde Chemistry Maps an Orthogonal Lysine Reactivity Landscape