PXD067595-1

PXD067595 is an original dataset announced via ProteomeXchange.

Title	Alterations in proteome and proteostasis networks during replicative senescence
Description	The accumulation of senescent cells constitutes a pivotal hallmark of tissue ageing, contributing to disruption of tissue homeostasis and thus, to the development of several aging-related diseases. Despite this, our knowledge about the molecular, spatial and temporal changes that drive aging on a cellular basis remains poorly understood. To elucidate these mechanisms, we employed mass spectrometry-based proteomics to determine changes in the total proteome, the ubiquitin-modified proteome, the phospho-proteome and in protein solubility during replicative senescence in human IMR90 fibroblasts. This accession contains the proteome data, across 4 distinct time points, with consecutive increasing concentrations of senescent cells.
HostingRepository	PRIDE
AnnounceDate	2026-07-16
AnnouncementXML	Submission_2026-07-16_08:06:45.632.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Petra Beli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	N-ethylmaleimide derivatized cysteine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-22 00:59:38	ID requested
⏵ 1	2026-07-16 08:06:46	announced

Dataset with its publication pending

submitter keyword: replicative senescence,IMR90 model, proteome

Petra Beli
contact affiliation	Institute of Molecular Biology (IMB), 55128 Mainz, Germany; Institute of Developmental Biology and Neurobiology (IDN), Johannes Gutenberg University, 55128 Mainz, Germany
contact email	p.beli@imb-mainz.de
lab head
Petra Beli
contact affiliation	Institute of Molecular Biology (IMB), Mainz, Germany
contact email	p.beli@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD067595

PRIDE project URI

• PRIDE
  1. PXD067595
     1. Label: PRIDE project
     2. Name: Alterations in proteome and proteostasis networks during replicative senescence