PXD067587-1

PXD067587 is an original dataset announced via ProteomeXchange.

Title	Systematic Evaluation of the Impact of Lysis Buffer on the Cellular Proteome and Glycosylation Modifications
Description	In this study, the effects of four commonly used lysis buffers (sodium dodecyl sulfate (SDS), guanidine hydrochloride (GuHCl), urea (UA), and Mammalian Protein Extraction Reagent (MPER)) on proteome and N-glycosylation modification analysis, providing valuable reference for optimizing experimental workflows in proteomics and glycoproteomics research. Proteins were digested into peptides using the Filter-Aided Sample Preparation (FASP) protocol, followed by enrichment of glycopeptides via Hydrophilic Interaction Liquid Chromatography (HILIC). Proteomic data were analyzed with MaxQuant, while glycoproteomic data were processed using pGlyco3 and Panda software.
HostingRepository	iProX
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_18:48:20.688.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bo Meng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 18:45:13	ID requested
⏵ 1	2025-08-21 18:48:21	announced

Dataset with its publication pending

submitter keyword: Proteomics, N-glycosylatio, SDS, GuHCl, MPER, UA

Yang Zhao
contact affiliation	National Insititute of Metrology
contact email	zhaoy@nim.ac.cn
lab head
Bo Meng
contact affiliation	National Institute of Metrology
contact email	meng920816@foxmail.com
dataset submitter

iProX dataset URI