PXD067575-1

PXD067575 is an original dataset announced via ProteomeXchange.

Title	LC-MS analysis of MPND associated complexes in H1299 cells
Description	he Mpr1/Pad1 N-terminal domain-containing protein (MPND) belongs to the JAMM family of deubiquitinases and is ubiquitously expressed across human tissues. However, its role in non-small cell lung cancer (NSCLC) remains unexplored. In this study, we demonstrate that MPND expression is frequently reduced in NSCLC, and that low levels of MPND correlate with poor clinical outcomes. Depletion of MPND in vitro enhances cell migration, invasion, and cancer stem cell (CSC)-like characteristics in NSCLC cells. Mechanistically, MPND interacts with histones, removing ubiquitin from H2A K119 monoubiquitination (H2A K119ub) and H2B K120 monoubiquitination (H2B K120ub). Loss of MPND elevates these ubiquitination marks, leading to alterations in chromatin architecture. Furthermore, MPND influences gene transcription, and its depletion activates the TGF-beta/SMAD3 signaling pathway. Specifically, knockout of MPND increases H2B K120 ubiquitination and chromatin accessibility at the SMAD3 locus, facilitating its transcriptional activation. Using mouse tumor models and clinical samples from NSCLC patients, we show that loss of MPND triggers activation of the TGF-beta/SMAD3 axis, thereby promoting tumor growth and metastasis. Collectively, these findings deepen our understanding of the epigenetic mechanisms underlying NSCLC progression and suggest that anti-TGF-beta therapies may be particularly beneficial for treating MPND-deficient tumors.
HostingRepository	PRIDE
AnnounceDate	2026-05-15
AnnouncementXML	Submission_2026-05-14_22:32:55.872.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dawei Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 08:48:28	ID requested
⏵ 1	2026-05-14 22:32:56	announced

Dataset with its publication pending

submitter keyword: complexes,MPND

Dawei Li
contact affiliation	Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University, Suzhou Medical College of Soochow University
contact email	daweili@suda.edu.cn
lab head
Dawei Li
contact affiliation	The Affiliated Zhangjiagang Hospital of Soochow University, Suzhou Medical College of Soochow University
contact email	daweili@suda.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067575

PRIDE project URI

• PRIDE
  1. PXD067575
     1. Label: PRIDE project
     2. Name: LC-MS analysis of MPND associated complexes in H1299 cells