PXD067564 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A subset of type 4 secretion system effectors of Brucella spp. associates to outer membrane vesicles |
| Description | The establishment of a replicative niche in the hostile environment of the host presents an enormous challenge for pathogens. Intracellular pathogens such as Brucella spp., the Gram-negative causative agents of Brucellosis, must subvert diverse host functions to ensure survival and replication. To schieve this, Brucella translocates effector proteins into host cells via its type 4 secretion system (T4SS), a key virulence factor. Here, we demonstrate that a subset of previously described effector proteins associates with outer membrane vesicles (OMVs) in different Brucella species. Most of these effector proteins encode predicted signal peptides for periplasmic export or transmembrane domains. Among them, BspC and VceA carry functional signal peptides that direct their export into the periplasm in a Sec-dependent manner. From the periplasm these proteins are subsequently secreted into the extracellular milieu, likely via the formation and release of OMVs. Our findings provide new insights into protein secretion by Brucella, suggesting that OMVs may represent an alternative secretion pathway to the T4SS. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-05 |
| AnnouncementXML | Submission_2026-01-05_02:33:37.526.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Naadir Ganief |
| SpeciesList | scientific name: Brucella abortus 2038; NCBI TaxID: NEWT:1310173; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-21 06:34:00 | ID requested | |
| ⏵ 1 | 2026-01-05 02:33:38 | announced | |
Publication List
Keyword List
| submitter keyword: PASEF, OMVs, Shotgun,Brucella, DDA |
Contact List
| Christoph Dehio |
|---|
| contact affiliation | Biozentrum, University of Basel, Basel, Switzerland |
| contact email | christoph.dehio@unibas.ch |
| lab head | |
| Naadir Ganief |
|---|
| contact affiliation | Biozentrum, University of Basel |
| contact email | naadir.ganief@unibas.ch |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD067564 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067564
- Label: PRIDE project
- Name: A subset of type 4 secretion system effectors of Brucella spp. associates to outer membrane vesicles
