PXD067549 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Hepatocyte mitochondrial NAD+ content is limiting for liver regeneration |
| Description | Supplemental NAD+ precursors show metabolic and functional benefits in rodent models of disease and are being explored as potential therapeutics in human studies. However, the wide range of processes that involve NAD+ in every cell and subcellular compartment make it difficult to narrow down mechanisms of action. Here we provide evidence that for a well-established benefit of NAD+ precursors in mice, faster liver regeneration, nearly the entire effect can be attributed to the concentration of NAD+ in hepatocyte mitochondria. We find that mitochondrial NAD+ concentration in hepatocytes of male mice is determined by the expression of the transporter SLC25A51 (MCART1), as has previously been shown in cultured cells. Heterozygous loss of SLC25A51 modestly decreases mitochondrial NAD+ content in multiple tissues and impairs liver regeneration, while hepatocyte-specific overexpression is sufficient to enhance regeneration comparably to the effect of systemic supplements, despite increasing NAD+ only in mitochondria. Thus, the hepatocyte mitochondrial NAD+ pool is a key determinant of the rate of liver regeneration. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-06 |
| AnnouncementXML | Submission_2025-11-05_18:04:33.330.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kevin A Janssen |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-21 05:12:27 | ID requested | |
| ⏵ 1 | 2025-11-05 18:04:33 | announced | |
Publication List
Keyword List
| submitter keyword: Mitochondria,Mouse, Liver, LC-MS/MS |
Contact List
| Douglas Wallace |
|---|
| contact affiliation | Center for Mitochondrial and Epigenomic Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA |
| contact email | wallaced1@chop.edu |
| lab head | |
| Kevin A Janssen |
|---|
| contact affiliation | Children's Hospital of Philadelphia |
| contact email | kxjanssen@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067549
- Label: PRIDE project
- Name: Hepatocyte mitochondrial NAD+ content is limiting for liver regeneration
