PXD067545-1

PXD067545 is an original dataset announced via ProteomeXchange.

Title	Protein interactions and phosphorylation status of Maf1 during optimal growth and stress conditions in the human parasite Leishmania major
Description	Maf1 was originally described as a repressor of RNA polymerase III transcription in response to stress conditions in yeast. Recent work has shown that Maf1 is involved in several functions across eukaryotes. Here we characterize Maf1 in Leishmania major (LmMaf1), a protozoan parasite that exhibits unconventional mechanisms of gene expression. Despite sequence divergence, LmMaf1 contains the conserved domains A, B and C. Four phosphorylated residues were detected in domains B and C in mid-logarithmic phase parasites by mass spectrometry. This is an unexpected result, since phosphorylation has not been reported in these domains in other organisms. Notably, no phosphorylated amino acids were found in stationary phase parasites, suggesting that nutrient stress might induce dephosphorylation of LmMaf1. Indirect immunofluorescence assays with a cell line that expresses LmMaf1 fused to a PTP tag showed that Maf1 mainly localizes to the nucleus. Interestingly, unlike other species, the LmMaf1 subcellular localization did not change in heat-shocked and stationary-phase parasites. Tandem affinity purifications were executed to identify the proteins that interact with LmMaf1 under optimal growth and heat stress. Although similar groups of proteins were identified in both normal and heat-stressed cells, the relative abundance of several of them showed important changes. For example, most RNA polymerase subunits increased significantly their relative abundance after heat shock. Altogether, these results strongly suggest the involvement of Maf1 in diverse roles that help to maintain general cellular homeostasis both under normal and stressful conditions in L. major, including transcription control, post-transcriptional regulation of mRNA expression, lipogenesis, and ribosomal biogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-11-24
AnnouncementXML	Submission_2025-11-24_09:19:43.483.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD067545
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ebbing de Jong
SpeciesList	scientific name: Leishmania major strain Friedlin; NCBI TaxID: NEWT:347515;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 04:57:53	ID requested
⏵ 1	2025-11-24 09:19:44	announced

10.1016/J.ACTATROPICA.2025.107882;

10.6019/PXD067545;

submitter keyword: Leishmania

Maf1

transcription

tRNA

heat stress

nutrient stress

Santiago Calvillo-Martinez
contact affiliation	Facultad de Estudios Superiores Iztacala, Unidad de Biomedicina, Universidad Nacional Autonoma de Mexico, Mexico
contact email	scalv@unam.mx
lab head
Ebbing de Jong
contact affiliation	SUNY Upstate Medical University
contact email	dejonge@upstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067545
     1. Label: PRIDE project
     2. Name: Protein interactions and phosphorylation status of Maf1 during optimal growth and stress conditions in the human parasite Leishmania major