PXD067529-1

PXD067529 is an original dataset announced via ProteomeXchange.

Title	Evaluating the impact of two different diets on the protein profile expression of the brain, liver, and intestine of the barramundi.
Description	This study investigates the impact of two commercial diets referred to as diet A and diet B on protein expression in the brain, liver, and intestine of barramundi. The diets were very similar in terms of crude fat and protein content, but diet B contained more fish meal and less protein from land animals. The feeding trial was conducted over 12 weeks to assess how differences in diet content influence fish health and growth at a molecular level. Throughout the trial, fish were fed twice daily, and water quality (including oxygen, salinity, ammonia, and pH) were monitored and adjusted daily. Proteins were extracted using a modified RIPA buffer and quantitative proteomic analysis was performed via data-independent acquisition (DIA) on a Q-Exactive HF-X mass spectrometer. Data was processed using DIA-NN and FragPipe. In order to validate findings from the proteomic data analysis which indicated effects on iron metabolism, iron concentrations in the tissues were measured using inductively coupled plasma–mass spectrometry (ICP-MS). These commercial diets serve as a nutritional benchmark for evaluating potential future feed formulations, such as the fermented tuna trimming fish meal. By first establishing a baseline with commonly used commercial feeds, this chapter sets the foundation for comparing the effectiveness and nutritional value of novel, sustainable protein sources for barramundi.
HostingRepository	jPOST
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_17:43:15.107.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohadeseh Montazeri Shatouri
SpeciesList	scientific name: Lates calcarifer; NCBI TaxID: 8187;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-20 22:35:26	ID requested
⏵ 1	2025-08-24 17:43:15	announced

Dataset with its publication pending

submitter keyword: Proteomics, Commercial feed, Aquaculture, Barramundi, Brain, Liver, Intestine, Diet

Professor Paul A Haynes
lab head
Mohadeseh Montazeri Shatouri
contact affiliation	Macquarie University
dataset submitter

jPOST dataset URI

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