PXD067516-1

PXD067516 is an original dataset announced via ProteomeXchange.

Title	Supporting data for manuscript: A modified fully automated dried blood spot sample preparation system and its application in steroid esters detection
Description	This project focuses on developing an efficient and sensitive method for detecting steroid esters in dried blood spots (DBS) to support anti-doping efforts. By optimizing a fully automated DBS processing system with an independent desorption pump, we established a high-throughput screening method for 30 steroid esters and a confirmatory method for 26 targets using HPLC-HRMS. The method demonstrates low detection limits (as low as 0.05 ng/mL) and good reproducibility (RSD < 15%), and its performance was validated through blind samples, administration studies, and comparison with traditional IRMS. This approach provides a practical solution for rapid and accurate screening and confirmation of steroid esters, enhancing anti-doping capabilities.
HostingRepository	iProX
AnnounceDate	2025-08-20
AnnouncementXML	Submission_2025-08-19_23:45:17.542.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Jing
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-19 23:44:08	ID requested
⏵ 1	2025-08-19 23:45:17	announced

Dataset with its publication pending

submitter keyword: dried blood spot

full automated analysis

steroid esters

doping testing

liquid chromatography–mass spectrometry

Xu Xin
contact affiliation	Shanghai University of Sport
contact email	xuxin@sus.edu.cn
lab head
Jing Jing
contact affiliation	Shanghai University of Sport
contact email	jing983699678@163.com
dataset submitter

iProX dataset URI