PXD067512-1

PXD067512 is an original dataset announced via ProteomeXchange.

Title	Ubiquitinated Protein Validation by PRM Analysis in SMG vs SO of E. tenella
Description	Eimeria tenella is highly host-specific and presents a monoxenous life cycle with multiple developmental stages. Each stage of E. tenella has different morphology, pathogenesis, gene and protein expressions which may change through gene regulatory and post-translational modifications (PTMs). Ubiquitination modulates a wide range of cellular functions and plays an important role in diverse cellular activities. Hitherto, there is no information on ubiquitination in E. tenella except inexact report on the enzymes related to ubiquitination. Dissecting the regulatory mechanism of E. tenella stage-conversion of ubiquitin is essential to understand parasite development. In this study, We used the ubiquitinated protein of SMG and SO stages to validate the proteomic data using PRM analysis. Skyline (v. 21.1) software was used for the alignment of the retention times, the peak detection of the peptide fragments and their quantification. We selected 14 ubiquitinated protein sites in 13 proteins for PRM validation, including L-dopachrome isomerase, glyceraldehyde-3-phosphate dehydrogenase, putative GRA9 protein, elongation factor 1-alpha, sag13, histone H2B, putative L-lactate dehydrogenase, putative polyubiquitin, putative myosin light chain TgMLC1, DNA-directed RNA polymerase II, putative myosin A, putative UV excision repair protein rhp23, and putative DNA damage inducible protein. We found that there is consistency in the SMG/SO trend by using the previous 4D label free method and PRM technique.
HostingRepository	iProX
AnnounceDate	2025-08-20
AnnouncementXML	Submission_2025-08-19_20:11:51.686.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zigang Qu
SpeciesList	scientific name: Eimeria tenella; NCBI TaxID: 5802;
ModificationList	ubiquitinylated lysine
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-19 20:11:29	ID requested
⏵ 1	2025-08-19 20:11:52	announced

Dataset with its publication pending

submitter keyword: 4D label free , E. tenella, PRM analysis, sporulated oocyst, The second-generation merozoites, Ubiquitination

Zigang Qu
contact affiliation	Lanzhou veterinary research institute, Chinese Academy of Agricultural Sciences
contact email	quzigang@caas.cn
lab head
Zigang Qu
contact affiliation	Lanzhou veterinary research institute, Chinese Academy of Agricultural Sciences
contact email	quzigang@caas.cn
dataset submitter

iProX dataset URI