PXD067507-1

PXD067507 is an original dataset announced via ProteomeXchange.

Title	Functions of the three common fungal extracellular membrane (CFEM) domain-containing genes of Arthrobotrys flagrans in the process of nematode trapping
Description	Arthrobotrys flagrans, a typical nematode-trapping fungus (NTF) that produces a three-dimensional adhesive network to capture nematodes, has excellent potential for the de-velopment of biocontrol agents against both plant and animal parasitic nematodes. Proteins containing the common fungal extracellular membrane (CFEM) domain are important for the nematodes' trapping by A. flagrans. The loss of AfCFEM1 and AfCFEM3 resulted in a significant upregulation of proteins associated with fungal pathogenicity, forming a denser adhesive material on the trap surface and ultimately increasing nematode mortality. However, the disruption of AfCFEM2 led to the opposite result. Furthermore, the deletion of AfCFEM1-3 not only affected trap morphology, resulting in an increased proportion of irregular traps (i.e., trap cells not fused to the hyphae), but also led to a thinner cell wall of the traps. Besides, the compensate effects among the CFEM family and other families were demonstrated. This study revealed that the AfCFEM1-3 genes in A. flagrans participated in nematode adhesion, cell wall formation, and intercellular communication, providing new insights into the functions of AfCFEM in the process of nematode trapping by NTF.
HostingRepository	iProX
AnnounceDate	2025-08-20
AnnouncementXML	Submission_2025-08-31_20:45:44.393.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tingting Shi
SpeciesList	scientific name: Arthrobotrys flagrans; NCBI TaxID: 97331;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-19 19:52:33	ID requested
⏵ 1	2025-08-31 20:45:44	announced

Dataset with its publication pending

submitter keyword: Nematode-trapping fungi, Caenorhabditis elegans, Adhesive material, CFEM, Pathogenicity

Guohong Li
contact affiliation	School of Science
contact email	ligh@ynu.edu.cn
lab head
Tingting Shi
contact affiliation	Yunnan University
contact email	940649541@qq.com
dataset submitter

iProX dataset URI