PXD067493-1

PXD067493 is an original dataset announced via ProteomeXchange.

Title	Optimized light supply strategy comprehensively increases the synthesis of high-value cell inclusions in Rhodopseudomonas palustris using food waste fermentation broth: Process optimization and biosynthetic mechanism
Description	The bioconversion of purple non-sulfur photosynthetic bacteria (PNSB) based on real food waste (FW) fermentation broth is crucial for FW resource recovery. This study enhanced the bioconversion efficiency of FW fermentation broth by PNSB through light intensity and photoperiod optimization, while elucidating the synthesis mechanisms of high-value cell inclusions. The results demonstrated that 4500 lx-L/D = 16/8 significantly enhanced R. palustris SYJ-1’s organic/nitrogen utilization efficiency and intracellular product synthesis. Proteomic analysis revealed that 4500 lx promoted the synthesis of geranylgeranyl pyrophosphate (GGPP), a precursor for photosynthetic pigments and coenzyme Q10 (CoQ10), increasing the contents of carotenoids, bacteriochlorophyll, and CoQ10 by 3.31-, 2.86-, 3.03-fold, respectively. Dark stimulation alleviated light-induced metabolic constraints by enhancing GGPP conversion and 4-hydroxybenzoate (another precursor of CoQ10) synthesis/modification, further promoting the production of these compounds. This study significantly advances the understanding of intracellular products synthesis in PNSB and provides a novel feasible approach for FW resource recovery.
HostingRepository	iProX
AnnounceDate	2025-08-19
AnnouncementXML	Submission_2025-08-18_20:22:54.773.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yujie Sun
SpeciesList	scientific name: Rhodopseudomonas palustris; NCBI TaxID: 1076;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-18 20:22:29	ID requested
⏵ 1	2025-08-18 20:22:55	announced

Dataset with its publication pending

submitter keyword: Non-sulfur photosynthetic bacteria, Light intensity, Photoperiod, High-value cell inclusion synthesis mechanism

Yujiao Sun
contact affiliation	Beijing Normal University
contact email	sunyujiao@bnu.edu.cn
lab head
Yujie Sun
contact affiliation	Beijing Normal University
contact email	1915198440@qq.com
dataset submitter

iProX dataset URI