PXD067466-1

PXD067466 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic analysis of Bacteroides thetaiotaomicron and Bacteroides acidifaciens following cultivation on hyaluronan and a standard substrate
Description	This project investigates proteomic adaptations of Bacteroides thetaiotaomicron and Bacteroides acidifaciens in response to hyaluronan as the sole glycosaminoglycan carbon source. Both species were cultivated in a standard medium and in a hyaluronan-based minimal medium, and their proteomes were analyzed by LC-MS/MS. The study aimed to identify global shifts in protein expression between growth conditions and to pinpoint specific proteins involved in hyaluronan degradation pathways. Comparative analysis between the two cultivation conditions provides insight into how gut-associated Bacteroides species regulate metabolic and enzymatic machinery for glycosaminoglycan utilization.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_20:05:58.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Roudnický
SpeciesList	scientific name: Bacteroides thetaiotaomicron; NCBI TaxID: NEWT:818; scientific name: Bacteroides acidifaciens; NCBI TaxID: NEWT:85831;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-18 06:44:51	ID requested
⏵ 1	2026-02-22 20:06:00	announced

Publication List

10.1002/mbo3.70227;
Sindelar M, Kocurkova A, Simek M, Roudnicky P, Ambrozova G, Kubala L, Turkova K, Revisiting Hyaluronan Catabolism in Bacteroides: Pathway Conservation, Overlooked Proteins, and Predictive Accuracy. Microbiologyopen, 15(1):e70227(2026) [pubmed]

10.1002/mbo3.70227;

Sindelar M, Kocurkova A, Simek M, Roudnicky P, Ambrozova G, Kubala L, Turkova K, Revisiting Hyaluronan Catabolism in Bacteroides: Pathway Conservation, Overlooked Proteins, and Predictive Accuracy. Microbiologyopen, 15(1):e70227(2026) [pubmed]

Keyword List

submitter keyword: LC-MS, timsTOF Pro,Hyaluronan
Bacteroides thetaiotaomicron
Bacteroides acidifaciens
Glycosaminoglycan metabolism

submitter keyword: LC-MS, timsTOF Pro,Hyaluronan

Bacteroides thetaiotaomicron

Bacteroides acidifaciens

Glycosaminoglycan metabolism

Contact List

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Pavel Roudnický
contact affiliation	Masaryk University
contact email	p.roudnicky@mail.muni.cz
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD067466
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD067466

PRIDE project URI

Repository Record List

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