PXD067465-1

PXD067465 is an original dataset announced via ProteomeXchange.

Title	Cultured hippocampal neurons glial fibrillary acidic protein (GFAP)
Description	Despite their widespread use as a research model, a comprehensive, quantitative proteomic profile of the cultured hippocampal neurons has remained unexplored. Here, we provide the first global proteomic characterization of primary murine hippocampal neurons cultured for 14 days under near-physiological glucose conditions (2.5 mM). Using label-free mass spectrometry and the Total Protein Approach (TPA), we quantified over 5,500 proteins, revealing a metabolic signature consistent with oxidative phosphorylation and a neuron-specific proteome largely devoid of astrocytic markers. Unexpectedly, glial fibrillary acidic protein (GFAP) was highly abundant in pure neuronal cultures, and its knockdown impaired neuronal viability and morphology. These findings suggest a compensatory role for GFAP in neurons cultured without astrocytic support. Additionally, our data highlight significant underexpression of key synaptic proteins compared to hippocampal tissue, underscoring the limitations of neuron-only culture systems. This study establishes a new molecular reference for hippocampal neurons and offers insights into neuronal adaptation in simplified environments.
HostingRepository	PRIDE
AnnounceDate	2026-01-22
AnnouncementXML	Submission_2026-01-22_08:10:30.724.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD067465
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mariusz Fleszar
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-18 06:21:07	ID requested
⏵ 1	2026-01-22 08:10:31	announced

10.6019/PXD067465;

submitter keyword: proteomics, GFPA,Neurons, hippocamp, LC-MS

Mariusz Fleszar
contact affiliation	Omics Research Center, Wroclaw Medical University, 50-368 Wroclaw, Poland
contact email	mariusz.fleszar@umw.edu.pl
lab head
Mariusz Fleszar
contact affiliation	Omics Research Center, Wroclaw Medical Universit
contact email	fleszar.mariusz@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067465
     1. Label: PRIDE project
     2. Name: Cultured hippocampal neurons glial fibrillary acidic protein (GFAP)