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PXD067426-1

PXD067426 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRIME of PbAP2-TR and PbMORC at the trophozoite stage
DescriptionTo explore the interaction-partners of PbAP2-TR and PbMORC in the asexual blood stage of Plasmodium berghei, rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) was performed, which is a combination of chromatin immunoprecipitation and liquid chromatography tandem-mass spectrometry. Parasites expressing GFP-fused PbAP2-TR and PbMORC were each generated from the Cas9-expressing parasite, and pbap2-g was then disrupted. Whole blood was harvested from mice infected with this transgenic parasite at 12 h post infection,and the parasites were then subjected to RIME experiments using anti-GFP antibody. Same procedures were performed for wild-type parasites as a control.
HostingRepositoryjPOST
AnnounceDate2025-12-08
AnnouncementXMLSubmission_2025-12-07_21:56:17.615.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD067426
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCentral Instrumentation Laboratory
SpeciesList scientific name: Plasmodium berghei; NCBI TaxID: 5821;
ModificationListL-methionine sulfoxide; deamidated L-glutamine; deamidated L-asparagine; unknown modification
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-08-17 23:34:19ID requested
12025-12-07 21:56:18announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plasmodium, transcription factor, chromatin remodeler
Contact List
Tsubasa Nishi
lab head
Central Instrumentation Laboratory
contact affiliationThe University of Osaka
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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