PXD067392 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In Vivo Characterization of Candida Extracellular Vesicles Reveals Unique Infection Pathway Proteins and Validates In Vitro Observations |
| Description | Extracellular vesicles (EVs) are membrane-bound particles secreted by cells, including microbes, and play key roles in intercellular communication and infection outcomes. Due to similarities between microbial and host EVs, isolating pathogen-specific EVs in vivo has been challenging. This study developed an immunoaffinity method using polyclonal antibodies against Candida albicans biofilm-derived EVs to selectively isolate fungal EVs from a rat catheter infection model. The method enabled specific capture and release of intact fungal EVs for analysis, without detecting host EVs. Characterization revealed EVs consistent with exosomes (~100 nm), and confirmed reduced EV production in a vesicle-defective Candida mutant (Hse1ΔΔ), validating in vitro findings. Proteomic analysis uncovered both previously known and novel Candida proteins, including the kinase CBK1, suggesting EV cargo adapts to in vivo conditions. This approach supports targeted EV study in infectious disease models and may aid in diagnostics and pathogenesis research. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-06 |
| AnnouncementXML | Submission_2026-04-06_08:59:04.298.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD067392 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Greg Sabat |
| SpeciesList | scientific name: Candida albicans SC5314; NCBI TaxID: NEWT:237561; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-15 12:06:15 | ID requested | |
| ⏵ 1 | 2026-04-06 08:59:04 | announced | |
Publication List
Keyword List
| submitter keyword: Candida, extracellular vesicles, infection, rat catheter infection model |
Contact List
| Justin Massey |
|---|
| contact affiliation | Infectious Disease, Department of Medicine, University of Wisconsin School of Medicine and Public Health |
| contact email | jmassey@medicine.wisc.edu |
| lab head | |
| Greg Sabat |
|---|
| contact affiliation | University of Wisconsin |
| contact email | gsabat@wisc.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD067392 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067392
- Label: PRIDE project
- Name: In Vivo Characterization of Candida Extracellular Vesicles Reveals Unique Infection Pathway Proteins and Validates In Vitro Observations
