PXD067376-1

PXD067376 is an original dataset announced via ProteomeXchange.

Title	Organellar LEA proteins ensure seed longevity by plastidial remodelling and embryonal degreening
Description	Seeds provide plant embryos with the remarkable ability to endure extended periods of times and extreme environmental conditions. Orthodox – or desiccation-tolerant – seeds, in particular, feature a number of adaptations to optimise resistance against extreme environments. Those traits must be established during seed maturation, since metabolism is reduced to a minimum during the desiccated phase and cannot support active defence. A characteristic of the maturation of most orthodox seeds is de-greening of the embryo, i.e. the regulated disassembly of the chloroplast thylakoid systems and chlorophyll degradation. While de-greening was shown to be under hormonal control, the physiological significance of thylakoid disassembly and reassembly remains unclear. Here we isolated an Arabidopsis lea48 lea42 line, as a model for depleted LEA function specifically in mitochondria and plastids of seeds. lea48 lea42 features a stay-green phenotype due to incomplete thylakoid degradation of the chloroplast, as evidenced by proteomic, transcriptomic and transmission electron microscopy analyses, yet without apparent signs of disrupted ABA homeostasis. While fresh lea48 lea42 seeds were unaffected in their germination properties, mutants were compromised in longevity at high-temperature/ high-humidity aging. Organelle-specific genetic complementation revealed that reduced longevity is linked to LEA ablation in the plastid, rather than the mitochondria. Strikingly, ‘light-ageing’, i.e. illumination of mature dry seeds, also reduced longevity delayed germination, which was associated with an accumulation of oxidised lipids. Our study pinpoints a novel role for LEA proteins in the regulated remodelling of seed mitochondria and plastids before desiccation. We identify embryo de-greening under the partial control of LEA as a key contributor to seed longevity, with direct evolutionary and ecological implications, as well as for strategies in plant breeding and agriculture.
HostingRepository	jPOST
AnnounceDate	2026-08-18
AnnouncementXML	Submission_2026-08-17_08:00:05.197.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jürgen Eirich
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-14 23:17:02	ID requested
⏵ 1	2026-08-17 08:00:05	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, seeds, LEA, mitochondria

Iris Finkemeier
lab head
Jürgen Eirich
contact affiliation	University of Münster - WWU - Institute for Biology and Biotechnology of Plants
dataset submitter

jPOST dataset URI

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