PXD067352-1

PXD067352 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling – LC/MS-MS with TurboID-ATG8e/atg2-1 Arabidopsis seedlings
Description	By employing a proximity labeling approach using Arabidopsis ATG8e tagged with TurboID as bait in atg2-1 background, we discovered a protein family of unknown function, consisting of two homologs (AT4G02880 and AT1G03290). Both proteins contain coil-coiled domains that are predicted to be closely related to the mammalian ELKS/Rab6-interacting/CAST (ERC) protein family, which acts as a central scaffold for forming a multidomain protein complex involved in synaptic vesicle secretion. As there is no available information about this protein family in plants, we have named these two proteins as ERC1 and ERC2, respectively.
HostingRepository	PRIDE
AnnounceDate	2025-10-07
AnnouncementXML	Submission_2025-10-07_11:53:28.722.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shouling Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-14 11:31:11	ID requested
⏵ 1	2025-10-07 11:53:29	announced

10.1101/2024.09.09.611939;

Chung KK, Zhao Z, Law KC, Ma J, Chiang CH, Leung KH, Shrestha R, Wu Y, Li C, Lee KM, Feng L, Li X, Wong KB, Xu SL, Gao C, Zhuang X, Biomolecular condensation of ERC1 recruits ATG8 and NBR1 to drive autophagosome formation for plant heat tolerance. bioRxiv, ():(2024) [pubmed]

submitter keyword: TurboID, Vesicle Secretion,AP-MS

Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
lab head
Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD067352

PRIDE project URI

• PRIDE
  1. PXD067352
     1. Label: PRIDE project
     2. Name: Proximity labeling – LC/MS-MS with TurboID-ATG8e/atg2-1 Arabidopsis seedlings