PXD067336-1

PXD067336 is an original dataset announced via ProteomeXchange.

Title	Oligodendrocyte precursor cell-specific blocking of low glucose-induced activation of AMPK ensures myelination and remyelination
Description	In this study, we screening the main types of cerebral cortex cells for glucose sensing and have find that AMPK is not activated in OPCs. We further discover that aldolase C (ALDOC) is acetylated at K14 in OPCs. Expression of ALDOC-K14R, with the acetylation lysine residues changed to arginine, renders OPCs able to activate AMPK in response to low glucose, and suppresses myelin regeneration in cuprizone, Lysolecithin (LPC) and EAE-induced demyelination model. But, genetic depletion of AMPKα1 and AMPKα2 in OPCs prevents the impact of ALDOC-K14R in remyelination. Furthermore, when OPCs activing AMPK with the TRPV antagonist AMG9810 or the hAMPKγ1D317A mutant, the proliferation and differentiation are both inhibited. Thus, our study reveals that ALDOC acetylation in OPCs plays a key role to prevent AMPK activation to endure its myelin repair function in neuronal disorders.
HostingRepository	iProX
AnnounceDate	2025-08-13
AnnouncementXML	Submission_2025-08-13_20:42:58.736.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chensong Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	acetylated residue; monomethylated residue; phosphorylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-13 20:42:36	ID requested
⏵ 1	2025-08-13 20:42:59	announced

Dataset with its publication pending

submitter keyword: Oligodendrocyte precursor cells (OPCs), Glucose, AMP dependent protein kinase (AMPK), Aldolase C (ALDOC), Acetylation, Myelination, Remyelination.

Chensong Zhang
contact affiliation	Xiamen University
contact email	cszhang@xmu.edu.cn
lab head
Chensong Zhang
contact affiliation	Xiamen University
contact email	cszhang@xmu.edu.cn
dataset submitter

iProX dataset URI