PXD067321-1

PXD067321 is an original dataset announced via ProteomeXchange.

Title	Metabolomic and proteomic differences in susceptible and benzimidazole-resistant adult females and males of Haemonchus contortus
Description	Anthelmintic resistance in parasitic nematodes, particularly in Haemonchus contortus, poses a significant threat to livestock health and productivity. Since resistance mechanisms have not yet been fully elucidated, the present study employed metabolomic and proteomic analyses of H. contortus adults (females and males separately) from drug-susceptible (ISE) and benzimidazole-resistant (IRE) strains, with a focus on resistance-specific differences. Using nuclear magnetic resonance (NMR)-based metabolomics and mass spectrometry-based proteomics, significant alterations in metabolic and protein expression profiles associated with resistance were identified. Resistant adults exhibited reduced levels of multiple amino acids and tricarboxylic acid cycle intermediates, alongside elevated levels of trehalose, myo-inositol, NAD+, and glycerophosphocholine, suggesting enhanced stress resistance and altered energy metabolism. Proteomic analysis revealed over 3000 proteins, with substantial sex-related differences. Moderate resistance-related differences in protein expression were observed, indicating adaptive biochemical pathways supporting resistance. While some resistance-associated changes in the metabolome and proteome were observed in both sexes, several were distinctly sex-specific. This finding provides the first evidence of such sex-dependent strategies in H. contortus, reinforcing the notion that anthelmintic resistance is a multifaceted and highly complex phenomenon, with many aspects yet to be fully understood. In addition, certain nematode-specific enzymes upregulated in the IRE strain -such as cysteine synthases and transthyretin-like proteins - might be considered as potential targets for future anthelmintic development.
HostingRepository	PRIDE
AnnounceDate	2026-01-02
AnnouncementXML	Submission_2026-01-02_01:37:25.201.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michaela Sadibolova
SpeciesList	scientific name: Haemonchus contortus (Barber pole worm); NCBI TaxID: NEWT:6289;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-13 14:02:49	ID requested
⏵ 1	2026-01-02 01:37:26	announced

Pelantov, á H, Š, adibolov, á M, Ž, ofka M, Matou, š, kov, á P, Luzarowski M, Kr, á, tk, ý J, Š, t, ě, rbov, á K, Kuzma M, Vos, á, la O, Sk, á, lov, á L, Metabolomic and proteomic differences in susceptible and benzimidazole-resistant adult females and males of Haemonchus contortus. Vet Res, 57(1):17(2025) [pubmed]

10.1186/s13567-025-01698-3;

submitter keyword: None

Petra Matouskova
contact affiliation	Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic
contact email	matousp7@faf.cuni.cz
lab head
Michaela Sadibolova
contact affiliation	University Hospital Hradec Kralove
contact email	michaela.sadibolova@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD067321

PRIDE project URI

• PRIDE
  1. PXD067321
     1. Label: PRIDE project
     2. Name: Metabolomic and proteomic differences in susceptible and benzimidazole-resistant adult females and males of Haemonchus contortus