PXD067256-1

PXD067256 is an original dataset announced via ProteomeXchange.

Title	The Koala (Phascolarctos cinereus) Prostate: A Proteomic Perspective on Gland Segmentation
Description	Australia’s most iconic animal, the koala (Phascolarctos cinereus), faces significant population decline from habitat loss, disease, and environmental pressures. Conservation has focused on assisted breeding technology and reproductive pathologies, yet koala reproductive biology remains poorly understood, particularly the accessory sex glands. The koala prostate has an important role both in the production of seminal plasma and in facilitating successful reproduction. Furthermore, prostatitis regularly occurs as a consequence of chlamydiosis, a substantial factor in the ongoing decline of koalas. This study presents the first proteomic profile of the koala prostate, offering insights into its histological segmentation and its broader functional significance. Prostate tissue from six mature male koalas was collected during breeding season, sampling the anterior and posterior segments. Proteins were digested using filter-aided sample preparation and analysed via LC-MS/MS with Zeno-SWATH acquisition. Peptide spectra were processed using DIA-NN and evaluated in RStudio™ to identify differentially expressed proteins and compare the koala prostate proteome with those of other species. Functional annotation and pathway analysis were performed using DAVID and Ingenuity Pathway Analysis, while secretory protein predictions were conducted using UniProt and SignalP. Segment-specific proteomic profiles revealed proteomic differentiation with secretory proteins contributing to segment-specific functions. Cross-species comparisons highlight strong homology between the koala and human prostate proteomes. This proteome provides a foundation for future investigations into prostate-related pathologies in koalas. Furthermore, Understanding the koala prostate at a molecular level helps advance wildlife conservation through a better comprehension of its role in male fertility and offers broader evolutionary insights into marsupial reproduction.
HostingRepository	PRIDE
AnnounceDate	2026-01-23
AnnouncementXML	Submission_2026-01-23_01:18:37.609.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yolande Campbell
SpeciesList	scientific name: Phascolarctos cinereus (Koala); NCBI TaxID: NEWT:38626;
ModificationList	iodoacetamide derivatized residue
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-12 04:09:20	ID requested
⏵ 1	2026-01-23 01:18:38	announced

Campbell Y, Johnston SD, Palmieri C, Pini T, The koala (Phascolarctos cinereus) prostate: a proteomic perspective on gland segmentation. Biol Reprod, 114(1):174-184(2026) [pubmed]

10.1093/biolre/ioaf209;

submitter keyword: koala, proteomics,Prostate

Taylor Pini
contact affiliation	University of Queensland, Vet School
contact email	t.pini@uq.edu.au
lab head
Yolande Campbell
contact affiliation	University of Queensland
contact email	yolande.campbell@uq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067256
     1. Label: PRIDE project
     2. Name: The Koala (Phascolarctos cinereus) Prostate: A Proteomic Perspective on Gland Segmentation