PXD067220-1

PXD067220 is an original dataset announced via ProteomeXchange.

Title	Effect of proteasome inhibition on total and poly-ubiquitylated proteomes of human A549 lung adenocarcinoma cells in different proliferation states
Description	Cellular stress responses are crucial for survival in sub-optimal conditions, and almost invar-iably involve proteome-wide alterations at the levels of protein abundance and post-translational modification with poly-ubiquitin chains (poly-ubiquitylation). However, most of our understanding of human cell stress responses at the protein level stems from experiments in rapidly-dividing cell culture models. Here, we used the A549 human diploid lung adenocarci-noma cell line (alveolar, type-2) in proliferating or bleomycin-induced senescent states, to ex-plore their responses to a severe proteotoxic stress with the proteasome inhibitor bortezomib (100 nM for 24 hours) at the total and poly-ubiquitylated proteome levels.
HostingRepository	PRIDE
AnnounceDate	2025-10-08
AnnouncementXML	Submission_2025-10-08_08:10:54.273.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harvey Johnston
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-11 14:48:38	ID requested
⏵ 1	2025-10-08 08:10:54	announced

10.1101/2025.09.07.674107;

submitter keyword: Human, Proteostasis, Insoluble, Lung, Cancer, Ubiquitin, Aggregate, LC-MSMS, Bortezomib, Stress, Senescence, Epithelia, Pro-teasome

Rahul Samant
contact affiliation	Signalling ISP, Babraham Institute, Cambridge, UK
contact email	rahul.samant@babraham.ac.uk
lab head
Harvey Johnston
contact affiliation	The Babraham Institute
contact email	harvey.johnston@babraham.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067220
     1. Label: PRIDE project
     2. Name: Effect of proteasome inhibition on total and poly-ubiquitylated proteomes of human A549 lung adenocarcinoma cells in different proliferation states