PXD067213-1

PXD067213 is an original dataset announced via ProteomeXchange.

Title	Proteomic and Phosphoproteomic Profiling of Heart in Pak2cko Mice Under Pressure Overload
Description	This study employs a factorial design to delineate the combinatorial effects of cardiac-specific conditional gene knockout and pressure overload-induced cardiac hypertrophy via transverse aortic constriction (TAC). Four experimental cohorts were established: (1) conditional knockout mice subjected to TAC (CKO_TAC), (2) knockout controls with Sham surgery (CKO_Sham), (3) floxed control mice receiving TAC (ff_TAC), and (4) floxed Sham-operated controls (ff_Sham). Following a 5-week pressure overload (TAC), myocardial tissue underwent parallel proteomic and phosphoproteomic profiling using TMT-based quantitative mass spectrometry. This dual-omics approach specifically interrogates: (a) the intrinsic genetic perturbation effect (CKO_ Sham vs ff_ Sham), (b) the pure pressure overload response (ff_TAC vs ff_Sham), and critically, (c) the genotype-by-environment interaction through synergistic/antagonistic divergence between observed versus predicted additive effects in CKO_TAC relative to genetic and surgical controls. Phosphoproteomic data further resolve signaling pathway dynamics underlying phenotypic modulation.
HostingRepository	PRIDE
AnnounceDate	2025-09-25
AnnouncementXML	Submission_2025-09-25_00:22:00.744.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dan Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-11 13:57:55	ID requested
⏵ 1	2025-09-25 00:22:01	announced

Dataset with its publication pending

submitter keyword: phosphoproteome, TMT,Pak2cko mouce heart, proteome, LC-MS/MS

Xiaoqiu Tan
contact affiliation	Key Laboratory of Medical Electrophysiology of the Ministry of Education Medical Electrophysiological Key Laboratory of Sichuan Province Institute of Cardiovascular Research, Southwest Medical University
contact email	tanxiaoqiu@swmu.edu.cn
lab head
Dan Zhang
contact affiliation	Southwest Medical University
contact email	zzdd2022@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067213
     1. Label: PRIDE project
     2. Name: Proteomic and Phosphoproteomic Profiling of Heart in Pak2cko Mice Under Pressure Overload