PXD067212-1

PXD067212 is an original dataset announced via ProteomeXchange.

Title	Retron Eco2 breaks tRNAs for antiphage defense
Description	Retrons are prokaryotic reverse transcriptase systems that produce multicopy single-stranded DNA (msDNA), yet the principles by which they mediate antiviral defense remain largely unresolved. Here, we dissect the mechanism of Eco2, a minimal retron composed of a single reverse transcriptase–nuclease fusion protein. Cryogenic electron microscopy and hydrogen-deuterium exchange mass spectrometry reveal the structures and dynamics of a trimeric nucleoprotein complex assembled within a branched msDNA scaffold, which cages the TOPRIM nuclease. We show that the phage-encoded endonucleases DenB initiates msDNA degradation, thereby unblocking the nuclease active site. Activated Eco2 cuts tRNAs, resulting in translational shutdown for antiphage defense. We further identify ribosomal protein S1 as a putative RNA chaperone that associates with the msDNA precursor. These findings provide insights into the molecular mechanisms of minimal retrons and establish a structural basis for the engineering of Eco2.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_16:08:54.346.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-11 13:50:57	ID requested
⏵ 1	2026-02-22 16:08:55	announced

Jasnauskait, ė M, Juozapaitis J, Liegut, ė T, Grigaitis R, Skorupskait, ė A, Steinchen W, Mik, š, ys A, Truncait, ė L, Kazlauskait, ė K, Torres Jim, é, nez MF, Khochare S, Dudas G, Bange G, Malinauskait, ė L, Songailien, ė I, Pausch P, Structure and mechanism of antiphage retron Eco2. Nat Struct Mol Biol, 33(2):330-340(2026) [pubmed]

10.1038/s41594-026-01754-2;

submitter keyword: retron antiphage defense ribosomal protein S1

Patrick Pausch
contact affiliation	Vilnius univeristy, Life Science Center, EBML PI
contact email	patrick.pausch@gmc.vu.lt
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD067212

PRIDE project URI

• PRIDE
  1. PXD067212
     1. Label: PRIDE project
     2. Name: Retron Eco2 breaks tRNAs for antiphage defense