PXD067201-1

PXD067201 is an original dataset announced via ProteomeXchange.

Title	Whole cell proteomics of iNeurons and iDA neurons at day 23 from Control, ASAH1-/- and SMPD1-/-
Description	This experiment describes a quantitative proteomic analysis of whole-cell lysates from induced neurons (iN) and induced dopaminergic neurons (iDA) differentiated from human embryonic stem cells of three genotypes: control, ASAH1 knockout, and SMPD1 knockout. Triplicate biological samples were prepared and analyzed using TMTpro 18-plex labeling coupled to high-resolution Orbitrap mass spectrometry with FAIMS. Data were processed with target-decoy database searching, stringent FDR control, and reporter ion–based quantification. The resulting dataset enables comparative profiling of proteomic alterations associated with lysosomal dysfunction and neurodegeneration-relevant pathways in distinct neuronal subtypes.
HostingRepository	PRIDE
AnnounceDate	2025-08-12
AnnouncementXML	Submission_2025-08-12_04:39:47.078.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felix Kraus
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-11 08:58:09	ID requested
⏵ 1	2025-08-12 04:39:47	announced

Dataset with its publication pending

submitter keyword: PD, iN, iDA, lysosome dysfunction, neurodegeneration,TMTpro

J. Wade Harper
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA, USA
contact email	wade_harper@hms.harvard.edu
lab head
Felix Kraus
contact affiliation	Harvard Medical Sschool
contact email	felix_kraus@hms.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067201
     1. Label: PRIDE project
     2. Name: Whole cell proteomics of iNeurons and iDA neurons at day 23 from Control, ASAH1-/- and SMPD1-/-