PXD067191-1

PXD067191 is an original dataset announced via ProteomeXchange.

Title	Functional analyses of the histone-like A104R protein of African swine fever virus and of a homologous pseudogene product found in soft tick genomes
Description	African swine fever virus (ASFV) causes a fatal disease with up to 100% mortality in domestic pigs and wild boar (species Sus scrofa), leading to significant economic losses for swine production. Unlike other eukaryotic viruses, ASFV encodes a histone-like nucleic acid-binding protein, pA104R. The A104R gene is highly conserved and present in all ASFV isolates of different genotypes. Moreover, A104R-like sequences have been identified in the genomes of soft ticks, which can replicate and transmit ASFV. Using a virulent genotype IX field isolate from Kenya, we analyzed the importance of A104R for viral replication in a permissive wild boar cell line (WSL). In this study, we confirmed that A104R is not essential for in vitro replication of ASFV. Loss of A104R did not detectably affect viral DNA replication or RNA transcription, but led to a moderate reduction of virus titers and plaque sizes. Substitution of A104R by a similar ASFV-like element derived from the genome of an Ornithodoros moubata soft tick was not capable of rescuing the deletion mutant phenotype. In contrast, reintroduction of the authentic A104R open reading frame (ORF) into the deletion mutant fully restored wild-type virus growth properties. In accompanying studies, we verified the DNA binding activities of the ASFV-, and of the tick-derived A104R protein (pA104R), and performed mass spectrometric analyses of the pA104R interactome. These experiments revealed besides DNA-dependent co-precipitated proteins, also specific, DNA-independent protein-protein interactions of pA104R with other viral and cellular proteins.
HostingRepository	PRIDE
AnnounceDate	2026-03-04
AnnouncementXML	Submission_2026-03-04_01:46:24.369.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD067191
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katarzyna Dolata
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-11 04:48:08	ID requested
⏵ 1	2026-03-04 01:46:24	announced

10.3390/V18020272;

10.6019/PXD067191;

submitter keyword: A104R protein,African swine fever virus, African swine fever virus-like integrated (ASFLI)-elements, A104R interactome

Katarzyna Dolata
contact affiliation	Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald-Insel Riems,Germany
contact email	katarzyna.dolata@fli.de
lab head
Katarzyna Dolata
contact affiliation	Friedrich-Loeffler Institut
contact email	katarzyna.dolata@fli.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067191
     1. Label: PRIDE project
     2. Name: Functional analyses of the histone-like A104R protein of African swine fever virus and of a homologous pseudogene product found in soft tick genomes