PXD067155-1

PXD067155 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Mouse Cerebral Cortex Following Experimental Ischemic Stroke: Identifying Novel Biomarkers of Damage and Repair
Description	Stroke remains a major global health challenge due to its high mortality and significant socioeconomic burden. Despite advances in clinical management, effective diagnostic tools and therapeutic strategies remain limited. This study aimed to identify and expand the repertoire of biomarkers of damage and repair that could serve as potential diagnostic and prognostic tools across post-stroke phases. Twenty-three male wild-type mice were assigned to control pre-stroke, 24-hour acute, and 35-day chronic post-stroke groups. Cerebral ischemic injury was induced via a 30-minute middle cerebral artery occlusion Koizumi method. Magnetic resonance imaging and neurological scoring were used to assess lesion size and functional deficit acutely, as well as structural and functional changes during the chronic phase. Proteomic profiling of the ipsilateral and contralateral cortices was performed using by data-independent acquisition (DIA)-based MS method. Seventy-four proteins showed significant alterations and were classified into four temporal expression clusters: acutely and chronically upregulated, acutely upregulated and chronically downregulated, acutely downregulated and chronically upregulated, and acutely and chronically downregulated. Gene ontology analysis identified 47 affected biological processes, including synaptic signaling, immune response, cell-cell communication, cytoskeletal organization, and proliferation. Several proteins not previously associated with stroke pathophysiology were identified, including 10 from the ipsilateral cortex (Dbi, Cpne3, Dnm2, Eef1a1, Taldo1, Pgls, Gnb5, Phf24, Ctsz, Capg) and 3 from the contralateral cortex (Agpat3, Cacng8, Endod). These findings provide novel molecular insights into post-stroke energy metabolism, neuroinflammation, and cellular remodeling, highlighting potential targets for further intervention.
HostingRepository	PRIDE
AnnounceDate	2026-01-19
AnnouncementXML	Submission_2026-01-18_17:34:12.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bruno Manadas
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	S-carboxamidoethyl-L-cysteine
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-09 15:12:34	ID requested
⏵ 1	2026-01-18 17:34:13	announced

Hamer D, Butorac A, Petrinec D, Berecki M, Mendes VM, Manadas B, Kelava V, K Hackenberger B, Glasnovi, ć A, Lovri, ć M, Gajovi, ć S, Dobrivojevi, ć, Radmilovi, ć M, Proteomic Analysis of Mouse Cerebral Cortex Following Experimental Ischemic Stroke: Identifying Novel Biomarkers of Damage and Repair. Cell Mol Neurobiol, 46(1):8(2025) [pubmed]

10.1007/s10571-025-01645-y;

submitter keyword: magnetic resonance imaging,mouse cerebral cortex, proteomic analysis, middle cerebral artery occlusion, ischemic injury, gene ontology

Srećko Gajović
contact affiliation	Croatian Institute for Brain Research and Department of Histology and Embryology, University of Zagreb School of Medicine, 10000 Zagreb, Croatia
contact email	srecko.gajovic@mef.hr
lab head
Bruno Manadas
contact affiliation	Center for Neurosciences and Cell Biology, University of Coimbra
contact email	bmanadas@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067155
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Mouse Cerebral Cortex Following Experimental Ischemic Stroke: Identifying Novel Biomarkers of Damage and Repair