PXD067153-1

PXD067153 is an original dataset announced via ProteomeXchange.

Title	Effect of heat-shock on total and poly-ubiquitylated proteomes of human A549 lung adeno-carcinoma cells in different proliferation states
Description	Cellular stress responses are crucial for survival in sub-optimal conditions, and almost invar-iably involve proteome-wide alterations at the levels of protein abundance and post-translational modification with poly-ubiquitin chains (poly-ubiquitylation). However, most of our understanding of human cell stress responses at the protein level stems from experiments in rapidly-dividing cell culture models. Here, we used the A549 human diploid lung adenocarci-noma cell line (epithelial, alveolar type-2) in proliferating or bleomycin-induced senescent states, to explore their responses to a severe heat-shock (44 degrees Celsius for 2 hours) at the total and poly-ubiquitylated proteome levels
HostingRepository	PRIDE
AnnounceDate	2025-10-08
AnnouncementXML	Submission_2025-10-08_07:53:07.328.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harvey Johnston
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-09 12:42:22	ID requested
⏵ 1	2025-10-08 07:53:07	announced

10.1101/2025.09.07.674107;

submitter keyword: Human, LC-MSMS, Proteostasis, Insoluble, Lung, Stress, Cancer, Senescence, Heat-Shock, Epithelia, Ubiquitin, Aggregate

Rahul Samant
contact affiliation	Signalling ISP, Babraham Institute, Cambridge, UK
contact email	Rahul.Samant@babraham.ac.uk
lab head
Harvey Johnston
contact affiliation	The Babraham Institute
contact email	harvey.johnston@babraham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD067153

PRIDE project URI

• PRIDE
  1. PXD067153
     1. Label: PRIDE project
     2. Name: Effect of heat-shock on total and poly-ubiquitylated proteomes of human A549 lung adeno-carcinoma cells in different proliferation states