PXD067139-1

PXD067139 is an original dataset announced via ProteomeXchange.

Title	Human lung cancer cells-MS for SNHG3 c.1746A>I editing
Description	Adenosine-to-inosine (A-to-I) RNA editing is a critical post-transcriptional modification that enhances tumor genome diversity and contributes to cancer progression. In non-small cell lung cancer (NSCLC), while specific A-to-I editing events have been identified, their functional mechanisms and clinical relevance remain poorly understood. Here, through whole-transcriptome analysis of NSCLC specimens, we discovered a hyper-editing event at position c.1746 in the long non-coding RNA SNHG3 (c.1746A>I), which correlates with advanced metastatic stages and reduced patient survival. Functional studies demonstrated that edited SNHG3 (SNHG3ED) exhibits significantly greater pro-metastatic activity compared to its wild-type counterpart (SNHG3WT). Mechanistically, SNHG3ED shows enhanced binding affinity for the chromatin remodeler SSRP1, triggering SSRP1-mediated replication origin assembly and subsequent upregulation of fatty acid metabolism and ferroptosis-related genes. This molecular rewiring promotes fatty acid oxidation, confers resistance to ferroptosis, and importantly, drives docetaxel (DTX) chemoresistance. In DTX-resistant NSCLC cell lines, patient-derived organoids and Nude mouse xenograft tumor model, antisense oligonucleotide-based targeting of SNHG3ED effectively restored DTX sensitivity and suppressed tumor growth. Our findings demonstrate that SNHG3 c.1746A>I editing serves both as a novel prognostic biomarker for NSCLC and as a mechanistically defined therapeutic target to overcome DTX resistance, which offers a potential therapeutic target to improve DTX efficacy.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:07:36.215.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-08 19:48:24	ID requested
⏵ 1	2025-09-14 16:07:37	announced

10.1038/s42003-025-08776-4;

Chen S, Zhuo A, Tang R, Su S, Wen B, Wei W, Xie J, Yu Z, Rao B, Lu J, Deng Y, Zhang Z, Yang L, A-to-I edited SNHG3 promotes non-small cell lung cancer metastasis by promoting fatty acid oxidation and resisting ferroptosis. Commun Biol, 8(1):1333(2025) [pubmed]

submitter keyword: Human, lung, SNHG3

Lei Yang
contact affiliation	Guangzhou Medical University
contact email	leiyang@gzhmu.edu.cn
lab head
Lei Yang
contact affiliation	Guangzhou Medical University
contact email	leiyang@gzhmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067139
     1. Label: PRIDE project
     2. Name: Human lung cancer cells-MS for SNHG3 c.1746A>I editing