PXD067099-1

PXD067099 is an original dataset announced via ProteomeXchange.

Title	High throughput tear proteomics with data independent acquisition enables biomarker discovery in allergic conditions
Description	The search for pathological biomarkers in biological fluids that can provide valuable insight into an individual’s health status, is a relevant area of research for multiple pathologies. Currently, the use of proteomics for the identification of differences in protein expression profiles between samples from healthy subjects and patients, has emerged as a powerful strategy to improve the current diagnosis of various pathologies or propose novel therapeutic approaches. Among the biological fluids from which new pathological biomarkers can be identified, tear secretion is highly attractive, since it can be collected non-invasively and could better concentrate proteins that sensitively reflect allergic responses, owing to their exposure to environmental factors and its connection to the respiratory system. Despite its potential, tear fluid remains underexplored, offering significant research opportunities. In this study, we collected human tear samples using the Shirmer Test from healthy and allergic individuals. Our optimized workflow, combining sample preparation and high-throughput proteomics using the data-independent acquisition (DIA) strategy, identified 2,542 proteins and enabled the successful differentiation of the two groups. We identified 99 differentially expressed proteins. Our results show the feasibility of protein analysis in human tear samples, highlighting tears as a highly sensitive fluid for detecting health conditions.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-25_08:44:56.635.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillermo Nourdin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-07 14:16:09	ID requested
⏵ 1	2025-08-25 08:44:57	announced

10.1038/S41598-025-17105-Y;

submitter keyword: diaPASEF., high-throughput proteomics,tear secretion, biomarkers

Karina Oyarce
contact affiliation	Laboratorio de Neuroinmunologia, Escuela de Tecnologia Medica, Facultad de Ciencias, Universidad San Sebastian, sede Concepcion, Concepcion, Chile.
contact email	Karina.oyarce@uss.cl
lab head
Guillermo Nourdin
contact affiliation	MELISA Institute
contact email	gnourdin@melisainstitute.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD067099

PRIDE project URI

• PRIDE
  1. PXD067099
     1. Label: PRIDE project
     2. Name: High throughput tear proteomics with data independent acquisition enables biomarker discovery in allergic conditions