PXD067098-1
PXD067098 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Microenvironmental signals combine to induce non-additive molecular and phenotypic responses in mammary epithelial cells |
| Description | The local microenvironment plays a critical role in determining cellular phenotype. While responses to many individual ligands have been well characterized, the integration of multiple extracellular signals into a cohesive molecular and phenotypic response remains largely unexplored. Here, we systematically investigate the combinatorial effects of Oncostatin M, Transforming Growth Factor Beta 1, and Epidermal Growth Factor on MCF10A mammary epithelial cells. Live-cell imaging revealed that ligand combinations produce emergent phenotypic responses distinct from single-ligand treatments, suggesting induction of new molecular programs. RNA sequencing showed synergistic upregulation of genes involved in migration and chemotaxis, including CXCL3 and CXCL5. Partial least squares regression identified transcriptional signatures associated with cellular phenotypes quantified from image data, which we validated in independent datasets. Finally, functional analysis revealed that synergistic upregulation of CXCR2 signaling, mediated by CREB activation, contributes to increased cell motility. This study provides a framework for understanding how complex ligand interactions shape phenotypic and molecular landscapes. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-08-07 |
| AnnouncementXML | Submission_2025-08-07_14:24:55.704.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Indranil Paul |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-07 14:12:15 | ID requested | |
| ⏵ 1 | 2025-08-07 14:24:56 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Cell Signaling, Microenvironment, Cell Migration, Pathway Integration, Cytokine, DatasetType:Proteomics |
Contact List
| Laura M. Heiser | |
|---|---|
| contact affiliation | Oregon Health and Science University |
| contact email | heiserl@ohsu.edu |
| lab head | |
| Indranil Paul | |
| contact affiliation | OHSU |
| contact email | pauli@ohsu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098762/ |
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