PXD067068-1

PXD067068 is an original dataset announced via ProteomeXchange.

Title	Leaf-age specific flood resilience in Arabidopsis thaliana is determined by distinct processes contributing to post-submergence recovery
Description	While the molecular mechanisms mediating submergence tolerance have been extensively studied, those underpinning age-dependent resilience remain poorly characterized. In Arabidopsis thaliana, submergence elicits a leaf-age dependent phenotype in which senescence and death progress across an age gradient starting with older leaves. Here we sought to investigate the mechanisms mediating this observed differential flood resilience by interrogating leaf age-specific transcriptome and proteome changes during submergence and recovery. Following submergence, most age-dependent differences were in the magnitude or speed of transcript abundance changes, whereas qualitative leaf-age dependent responses were most apparent during recovery. This included a strong desiccation response in old leaves despite a stronger ABA-signaling response. Physiological measurements suggested that faster dehydration was mediated by a combination of submergence-mediated reduction of ABA sensitivity and higher conductance in old leaves. We also observed a stronger induction in young shoot tissue of genes associated with endoplasmic reticulum (ER) stress and the unfolded protein response (UPR). Mutants disabled in the two UPR signaling branches were affected in new leaf formation and the ability to restore the proteome, but not in senescence, suggesting that young tissues activate ER stress recovery to permit continuation of growth. Of the mitochondrial membrane proteins differentially regulated in young leaves, loss of mitochondrial voltage-dependent anion channel function impacted submergence-recovery tolerance. Our data reveal multiple mechanisms underlying leaf age-dependent differential submergence recovery and demonstrate how tolerance is determined by an interplay between age related developmental traits and stress signaling pathways.
HostingRepository	PRIDE
AnnounceDate	2026-08-14
AnnouncementXML	Submission_2026-08-14_09:07:11.971.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongtao Zhang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-07 06:41:08	ID requested
⏵ 1	2026-08-14 09:07:12	announced

Rankenberg T, Sanclemente MA, Zhang H, Leeggangers HA, Orozco-Granados O, Devaiah MB, Chao JR, van Veen H, Theodoulou FL, Sasidharan R, Distinct processes contributing to post-submergence recovery determine leaf age-specific flood resilience in Arabidopsis. Plant Physiol, ():(2026) [pubmed]

10.1093/plphys/kiag317;

submitter keyword: Arabidopsis thaliana leaf proteome post submergence recovery TMTpro

Hongtao Zhang
contact affiliation	Rothamsted Research, Harpenden, AL5 2JQ, UK
contact email	Hongtao.zhang@rothamsted.ac.uk
lab head
Hongtao Zhang
contact affiliation	Rothamsted Research, Harpenden, AL5 2JQ, UK
contact email	hongtao.zhang@rothamsted.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067068
     1. Label: PRIDE project
     2. Name: Leaf-age specific flood resilience in Arabidopsis thaliana is determined by distinct processes contributing to post-submergence recovery