PXD067042 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparative phosphoproteomic analysis of HeLa cells in response to etoposide or to a combination of etoposide and the ATR inhibitor VE821 |
| Description | To investigate the regulation of protein phosphorylation during the DNA damage response and the role of ATR kinase activity, we performed a comparative phosphoproteomic analysis of HeLa cells treated with etoposide alone or in combination with the ATR inhibitor VE821. Etoposide is a topoisomerase II inhibitor that induces DNA double-strand breaks, activating the DNA damage signaling cascade. We focused particularly on phosphorylation events associated with Treacle (TCOF1), a nucleolar protein implicated in the regulation of rDNA transcription and nucleolar DNA damage response. Quantitative mass spectrometry-based phosphoproteomics was used to identify and quantify global phosphorylation changes under both treatment conditions. This dataset enables the identification of ATR-dependent phosphorylation sites and may provide insights into the molecular mechanisms underlying nucleolar stress responses and the coordination of DNA repair processes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-25 |
| AnnouncementXML | Submission_2026-05-24_16:28:22.225.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD067042 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Georgij Arapidi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-07 04:24:03 | ID requested | |
| ⏵ 1 | 2026-05-24 16:28:23 | announced | |
Publication List
| 10.1093/nar/gkag350; |
| Petrova NV, Deriglazov DA, Kovina AP, Luzhin AV, Shender VO, Arapidi GP, Gavrikov AS, Mishin AS, Razin SV, Velichko AK, Treacle-dependent TOPBP1 condensation regulates the nucleolar DNA damage response. Nucleic Acids Res, 54(9):(2026) [pubmed] |
| 10.6019/PXD067042; |
Keyword List
| submitter keyword: phosphoproteomics, etoposide, ATR inhibitor |
Contact List
| Georgij Arapidi |
|---|
| contact affiliation | System Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
| contact email | arapidi@gmail.com |
| lab head | |
| Georgij Arapidi |
|---|
| contact affiliation | System Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
| contact email | arapidi@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067042
- Label: PRIDE project
- Name: Comparative phosphoproteomic analysis of HeLa cells in response to etoposide or to a combination of etoposide and the ATR inhibitor VE821
