PXD067041-1

PXD067041 is an original dataset announced via ProteomeXchange.

Title	Outer membrane and Whole cell proteome Analysis of tamA, tamB and tamAB knockout mutants compared to the wildtype
Description	The Translocation and Assembly Module (TAM), composed of TamA and TamB, facilitates the insertion of some β-barrel proteins into the OM of Escherichia coli and Klebsiella pneumoniae, and has also been implicated in lipid homeostasis. However, its role in Pseudomonas aeruginosa remains mostly uncharacterized. To investigate TAM’s function and drug target potential in P. aeruginosa, we generated both single gene knockouts and the tamAB double knockout and isolated outer membrane proteins (OMP) and whole cell lysate (WCP) and run mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2025-09-16
AnnouncementXML	Submission_2025-09-16_05:54:31.487.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Boocock
SpeciesList	scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611;
ModificationList	iodoacetamide derivatized residue
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-07 04:13:59	ID requested
⏵ 1	2025-09-16 05:54:31	announced

Dataset with its publication pending

submitter keyword: SWATH,Pseudomonas aeruginosa, Outer membrane proteins

David Boocock
contact affiliation	John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham NG11 8NS
contact email	david.boocock@ntu.ac.uk
lab head
David Boocock
contact affiliation	Nottingham Trent University
contact email	david.boocock@ntu.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067041
     1. Label: PRIDE project
     2. Name: Outer membrane and Whole cell proteome Analysis of tamA, tamB and tamAB knockout mutants compared to the wildtype