PXD067034-1

PXD067034 is an original dataset announced via ProteomeXchange.

Title	Active zone plasticity drives presynaptic dephosphorylation in Drosophila
Description	The function of the nervous system relies on tightly regulated, yet plastic synaptic vesicle release machinery at the presynaptic active zone. In Drosophila, a form of presynaptic active zone plasticity, Presynaptic Scaling, or PreScale, is executed by the ELKS-family scaffold protein Bruchpilot (BRP), which drives the scaling of the Munc13 family release factors and other synaptic proteins. Genetic PreScale induction encodes sleep need and mediates early brain aging. However, the molecular mechanisms by which PreScale regulates sleep and brain aging remains unknown. Here, using an integrated-omics approach, we analyzed the proteome and phospho-proteome landscapes of genetic PreScale triggered by titrating brp gene copies from 1 to 4. Proteomic and bioinformatic analyses revealed changes in metabolic, immune, and stress responses pathways, as well as local translation control. Importantly, phospho-proteomic analysis showed BRP-induced PreScale provokes a global synaptic hypophosphorylation of proteins enriched for presynaptic components, suggesting a functional switch by reprogramming of the equilibrium of synaptic phosphorylation and dephosphorylation. Synaptic hypophosphorylation in PreScale is likely contributed by activity changes in Protein Kinase Aand the regulatory subunit of protein phosphatase 1 Spinophilin. We propose that synaptic hypophosphorylation might represent a typical molecular signature of PreScale in sleep homeostasis and brain aging, highlighting post-translation modifications in supporting long-term presynaptic plasticity.
HostingRepository	PRIDE
AnnounceDate	2026-06-09
AnnouncementXML	Submission_2026-06-09_03:03:02.033.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD067034
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laxmikanth Kollipara
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-07 02:13:27	ID requested
⏵ 1	2026-06-09 03:03:02	announced

10.1073/PNAS.2524065123;

10.6019/PXD067034;

submitter keyword: Bruchpilot (BRP), Phospho-proteomics,Drosophila, Presynaptic Scaling, PreScale, Proteomics

Prof. Dr. Albert Sickmann
contact affiliation	President ISAS e.V. Otto-Hahn-Str. 6b 44227 Dortmund, Germany P: +49 (0)2 31.13 92-100 albert.sickmann@isas.de www.isas.de LinkedIn: leibniz-institut-isas Bluesky: @isas-leibniz.bsky.social
contact email	albert.sickmann@isas.de
lab head
Laxmikanth Kollipara
contact affiliation	Leibniz – Institut für Analytische Wissenschaften - ISAS - e.V.
contact email	laxmikanth.kollipara@isas.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD067034

PRIDE project URI

• PRIDE
  1. PXD067034
     1. Label: PRIDE project
     2. Name: Active zone plasticity drives presynaptic dephosphorylation in Drosophila